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DNA-hydrolyzing IgG antibodies from the blood of patients with acquired immune deficiency syndrome

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Abstract

DNase activity was analyzed in 110 IgG preparations from the blood of AIDS patients. The relative activity of the preparations varied markedly among patients, being reliably detectable in 96% of the preparations. It was shown with several rigid criteria that DNAase activity is an intrinsic property of antibodies (Abs) from AIDS patients. Not only intact IgG, but also isolated light chains of polyclonal Abs were shown to possess catalytic activity. The abzymes efficiently catalyzed DNA hydrolysis in a wide range of pH (5.0–9.5). The K M and V max values were evaluated for Ab-dependent hydrolysis of DNA.

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References

  1. Tramontano A., Gololobov G., Paul S. 2000. Proteolytic antibodies: Origins, selection, and induction. Chem. Immunol. 77, 1–17.

    PubMed  CAS  Google Scholar 

  2. Gololobov G., Tramontano A., Paul S. 2000. Nucleophilic proteolytic antibodies. Appl. Biochem. Biotechnol. 83, 221–232, 297–313.

    Article  PubMed  CAS  Google Scholar 

  3. Nevinsky G.A., Buneva V.N. 2005. Natural catalytic antibodies-abzymes. In: Catalytic Antibodies. Ed. Keinan E., Weinheim: VCH-Wiley, pp. 503–567.

    Google Scholar 

  4. Nevinsky G.A., Favorova O.O., Buneva B.N. 2002. Catalytic antibodies: New characters in the protein repertoire. In: Protein-Protein Interactions. A Molecular Cloning Manual. Ed. Golemis E., Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press, pp. 523–534.

    Google Scholar 

  5. Nevinsky G.A., Buneva V.N. 2002. Human catalytic RNA-and DNA-hydrolyzing antibodies. J. Immunol. Methods. 269, 235–249.

    Article  PubMed  CAS  Google Scholar 

  6. Nevinsky G.A., Kanyshkova T.G., Buneva V.N. 2000. Natural catalytic antibodies (abzymes) in normalcy and pathology. Biokhimiya. 65, 1473–1487.

    Google Scholar 

  7. Nevinsky G.A., Semenov D.V., Buneva V.N. 2000. Catalytically active antibodies (abzymes) induced by chemically stable analogs of transitional states: A review. Biokhimiya. 65, 1459–1472.

    Google Scholar 

  8. Paul S., Volle D.J., Beach C.M., Johnson D.R., Powell M.J., Massey R.J. 1989. Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody. Science. 244, 1158–1162.

    Article  PubMed  CAS  Google Scholar 

  9. Shuster A.M., Gololobov G.V., Kvashuk O.A., Bogomolova A.E., Smirnov I.V., Gabibov A.G. 1992. DNA hydrolyzing autoantibodies. Science. 256, 665–667.

    Article  PubMed  CAS  Google Scholar 

  10. Andrievskaya O.A., Buneva V.N., Baranovskii A.G., Gal’vita A.V., Benzo E.S., Naumov V.A., Nevinsky G.A. 2002. Catalytic diversity of polyclonal RNA-hydrolyzing IgG antibodies from the sera of patients with systemic lupus erythematosus. Immunol. Lett. 81, 191–198.

    Article  PubMed  CAS  Google Scholar 

  11. Andrievskaia O.A., Buneva V.N., Zabara V.G., Naumov V.A., Iamkovoi V.I., Nevinsky G.A. 2000. Catalytic heterogeneity of polyclonal RNA-hydrolyzing IgM from sera of patients with lupus erythematosus. Med. Sci. Monit. 6, 460–470.

    Google Scholar 

  12. Buniva V.N., Andrievskaya O.A., Romannikova I.V., Gololobov G.V., Yadav R.P., Yamkovoi V.I., Nevinsky G.A. 1994. Interaction of catalytically active antibodies with oligoribonucleotides. Mol. Biol. 28, 738–743.

    Google Scholar 

  13. Kozyr A.V., Kolesnikov A.V., Aleksandrova E.S., Sashchenko L.P., Gnuchev N.V., Favorov P.V., Kotelnikov M.A., Iakhnina E.I., Astsaturov I.A., Prokaeva T.B., Alekberova Z.S., Suchkov S.V., Gabibov A.G. 1998. Novel functional activities of anti-DNA autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases. Appl. Biochem. Biotechnol. 75, 45–61.

    PubMed  CAS  Google Scholar 

  14. Vlassov A.V., Baranovskii A.G., Kanyshkova T.G., Prints A.V., Zabara V.G., Naumov V.A., Breusov A.A., Buneva V.N., Nevinsky G.A. 1998. Substrate specificity of DNA-and RNA-hydrolyzing antibodies from the blood of patients with polyarthritis and autoimmune thyroiditis. Mol. Biol. 32, 559–569.

    Google Scholar 

  15. Baranovskii A.G., Matyushin V.G., Vlassov A.V., Zabara V.G., Naumov V.A., Buneva V.N., Nevinsky G.A. 1997. DNA-and RNA-hydrolyzing antibodies from the blood of patients with different forms of viral hepatitis. Biokhimiya. 62, 1590–1599.

    Google Scholar 

  16. Savel’ev A.N., Eneyskaya E.V., Shabalin K.A., Michael V., Filatov M.V., Neustroev K.N. 1999. Antibodies with amylolytic activity. Protein Peptide Lett. 6, 179–184.

    CAS  Google Scholar 

  17. Neustoev K.N., Ivanen D.R., Eulminskaya A.A., Brumer I.H., Saveliev A.N., Nevinsky G.A. 2003. Amylolytic activity and catalytic properties of IgM and IgG antibodies from patients with systemic lupus erithromatosis. Hum. Antibodies. 12, 31–34.

    Google Scholar 

  18. Nevinsky G.A., Breusov A.A., Baranovskii A.G., Prints A.V., Kanyshkova T.G., Galvita A.V., Naumov V.A., Buneva V.N. 2001. Effect of different drugs on the level of DNA-hydrolyzing polyclonal IgG antibodies in sera of patients with Hashimoto’s thyroiditis and nontoxic nodal goiter. Med. Sci. Monit. 7, 201–211.

    PubMed  CAS  Google Scholar 

  19. Savel’ev A.N., Ivanen D.R., Kulminskaya A.A., Ershova N.A., Kanyshkova T.G., Buneva V.N., Mogelnitskii A.S., Doronin B.M., Favorova O.O., Nevinsky G.A., Neustroev K.N. 2003. Amylolytic activity of IgM and IgG antibodies from patients with multiple sclerosis. Immunol. Lett. 86, 291–297.

    Article  CAS  Google Scholar 

  20. Baranovskii A.G., Odintsova E.S., Buneva V.N., Doronin B.M., Nevinsky G.A. 2004. Comparison of enzymatic properties of DNA-abzymes and human DNAses. Nucleosides Nucleotides Nucleic Acids. 23, 1053–1056.

    Article  PubMed  CAS  Google Scholar 

  21. Baranovskii A.G., Ershova N.A., Buneva V.N., Kanyshkova T.G., Mogelnitskii A.S., Doronin B.M., Boiko A.N., Gusev E.I., Favorova O.O., Nevinsky G.A. 2001. Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis. Immunol. Lett. 76, 163–167.

    Article  PubMed  CAS  Google Scholar 

  22. Li L., Kaveri S., Tyutyulkova S., Kazatchkine M.D., Paul S. 1995. Catalytic activity of anti-thyroglobulin antibodies. J. Immunol. 154, 3328–3332.

    PubMed  CAS  Google Scholar 

  23. Kalaga R., Li L., O’Dell J.R., Paul S. 1995. Unexpected presence of polyreactive catalytic antibodies in IgG from unimmunized donors decreased levels in rheumatoid arthritis. J. Immunol. 155, 2695–2702.

    PubMed  CAS  Google Scholar 

  24. Thiagarajan P., Dannenbring R., Matsuura K., Tramontano A., Gololobov G., Paul S. 2000. Monoclonal antibody light chain with prothrombinase activity. Biochemistry. 39, 6459–6465.

    Article  PubMed  CAS  Google Scholar 

  25. Lacroix-Desmazes S., Moreau A., Sooryanarayana S., Bonnemain C., Stieltjes N., Pashov A., Sultan Y., Hoebeke J., Kazatchkine M.D., Kaveri S.V. 1999. Catalytic activity of antibodies against factor VIII in patients with hemophilia A. Nat. Med. 5, 1044–1047.

    Article  PubMed  CAS  Google Scholar 

  26. Polosukhina D.I., Kanyshkova T.G., Doronin B.M., Tyshkevich O.B., Buneva V.N., Boiko A.N., Gusev E.I., Favorova O.O., Nevinsky G.A. 2004. Hydrolysis of myelin basic protein by polyclonal catalytic IgGs from the sera of patients with multiple sclerosis. J. Cell Mol. Med. 8, 359–368.

    PubMed  CAS  Google Scholar 

  27. Polosukhina D.I., Buneva V.N., Doronin B.M., Tyshkevich O.B., Boiko A.N., Gusev E.I., Favorova O.O., Nevinsky G.A. 2005. Hydrolysis of myelin basic protein by IgM and IgA antibodies from the sera of patients with multiple sclerosis. Med. Sci. Monit. 11, BR266–BR272.

    PubMed  CAS  Google Scholar 

  28. Polosukhina D.I., Kanyshkova T.G., Doronin B.M., Tyshkevich O.B., Buneva V.N., Boiko A.N., Gusev E.I., Favorova O.O., Nevinsky G.A. 2006. Metal-dependent hydrolysis of myelin basic protein by IgGs from the sera of patients with multiple sclerosis. Immunol. Lett. 103, 75–81.

    Article  PubMed  CAS  Google Scholar 

  29. Nevinsky G.A. Kanyshkova T.G., Semenov D.V., Vlassov A.V., Gal’vita A.V., Buneva V.N. 2000. Secretory immunoglobulin A from healthy human mothers’ milk catalyzes nucleic acid hydrolysis. Appl. Biochem. Biotechnol. 83, 115–130, 145–153.

    Article  PubMed  CAS  Google Scholar 

  30. Buneva V.N., Kanyshkova T.G., Vlassov A.V., Semenov D.V., Khlimankov D.Yu., Breusova L.R., Nevinsky G.A. 1998. Catalytic DNA-and RNA-hydrolyzing antibodies from milk of healthy human mothers. Appl. Biochem. Biotechnol. 75, 63–76.

    PubMed  CAS  Google Scholar 

  31. Semenov D.V., Kanyshkova T.G., Karotaeva N.A., Krasnorutskii M.A., Kuznetsova I.A., Buneva V.N., Nevinsky G.A. 2004. Catalytic nucleotide-hydrolyzing antibodies in milk serum of clinically healthy human mothers. Med. Sci. Monit. 10, BR23–BR33.

    PubMed  CAS  Google Scholar 

  32. Sevenov D.V., Kanyshkova T.G., Kit Yu.A., Khlimankov D.Yu., Akimzhanov A.M., Gorbunov D.A., Buneva V.N., Nevinsky G.A. 1998. Human immunoglobulins G hydrolyze nucleotides. Biokhimiya. 63, 1097–1106.

    Google Scholar 

  33. Savel’ev A.N., Kanyshkova T.G., Kulminskaya A.A., Buneva, V.N., Eneyskaya E.V., Filatov M.V., Nevinsky G.A., Neustroev K.N. 2001. Amylolytic activity of IgG and sIgA immunoglobulins from human milk. Clin. Chim. Acta. 314, 141–152.

    Article  PubMed  CAS  Google Scholar 

  34. Nevinsky G.A., Kit Y.Ya., Semenov D.V., Khlimankov D.Yu., Buneva V.N. 1998. Secretory immunoglobulin A from human milk catalyzes milk protein phosphorylation. Appl. Biochem. Biotechnol. 75, 77–91.

    PubMed  CAS  Google Scholar 

  35. Kit Y., Semenov D.V., Nevinsky G.A. 1996. Phosphorylation of different human milk proteins by human catalytic secretory immunoglobulin A. Biochem. Mol. Biol. Int. 39, 521–527.

    PubMed  CAS  Google Scholar 

  36. Gorbunov D.V., Karataeva N.A., Buneva V.N., Nevinsky G.A. 2005. Lipid kinase activity of antibodies from milk of clinically healthy human mothers. Biochim. Biophys. Acta. 1735, 153–166.

    PubMed  CAS  Google Scholar 

  37. Karataeva N.A., Gorbunov D.V., Prokudin I.V., Buneva V.N., Kulminskaya A.A., Neustroev K.N., Nevinsky G.A. 2006. Human milk antibodies with polysaccharide kinase activity. Immunol. Lett. 103, 58–67.

    Article  PubMed  CAS  Google Scholar 

  38. Baranovskii A.G., Kanyshkova T.G., Mogil’nitskii A.S., Naumov V.A., Buneva V.N., Baiko A.N., Favorova O.O., Nevinsky G.A. 1998. Polyclonal antibodies from the blood of patients with multiple sclerosis effectively hydrolyze RNA and DNA. Biokhimiya. 63, 1459–1469.

    Google Scholar 

  39. Buneva V.N., Kudryantseva A.N., Gal’vita A.V., Dubrovskaya V.V., Khokhlova O.V., Kalinina I.A., Galenok V.A., Nevinsky G.A. 2003. Dynamics of nuclease activity of antibodies in the blood of pregnant and lactating women. Biokhimiya. 68, 1088–1100.

    Google Scholar 

  40. Denisenko V.B., Sizyakina L.P., Simovan’yan E.N., Shemshura A.B. 1999. Immunopathogenetic foundations of the diagnosis of HIV infection stages in children. Immunol. SPIDa. 2, 13–15.

    Google Scholar 

  41. Faucci A., Braunwald E., Isselbacher K., Wilson G., Martin J., Kasper D., Hauser S. 2002. Internal Diseases by Thisley R. Harrison [Russian translation]. Moscow: Praktika-McGraw-Hill, vol. 1.

    Google Scholar 

  42. Odintsova E.S., Buneva V.N., Nevinsky G.A. 2005. Casein-hydrolyzing activity of sIgA antibodies from human milk. J. Mol. Recognit. 18, 413–421.

    Article  PubMed  CAS  Google Scholar 

  43. Gololobov G.V., Mikhalap S.V., Starov A.V., Kolesnikov A.F., Gabibov A.G. 1994. DNA-protein complexes. Natural targets for DNA-hydrolyzing antibodies. Appl. Biochem. Biotechnol. 47, 305–315.

    PubMed  CAS  Google Scholar 

  44. Osterman L.A. 1981. Metody issledovaniya belkov i nukleinovykh kislot: elektroforez i tsentrifugirovanie (Methods of Protein and Nucleic Acids Research: Electrophoresis and Centrigfugation). Moscow: Nauka.

    Google Scholar 

  45. Merril C.R., Goldman D., Van Keuren M.L. 1984. Gel protein stains: Silver stain. Meth. Enzymol. 104, 441–447.

    Article  PubMed  CAS  Google Scholar 

  46. Towbin H., Staehelin T., Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76, 4350–4354.

    Article  PubMed  CAS  Google Scholar 

  47. Odintsova E.S., Kharitonova M.A., Baranovskii A.G., Sizyakina L.P., Buneva V.N., Nevinsky G.A. 2006. Proteolytic activity of IgG antibodies from the blood of AIDS patients. Biokhimiya. 71, 320–332.

    Google Scholar 

  48. Kanyshkova T.G., Semenov D.V., Khlimankov D.Yu., Buneva V.N., Nevinsky G.A. 1997. DNA-hydrolyzing activity of the light chain of IgG antibodies from milk of healthy human mothers. FEBS Lett. 416, 23–26.

    Article  PubMed  CAS  Google Scholar 

  49. Baranovskii A.G., Buneva V.N., Nevinsky G.A. 2004. Human deoxyribonucleases: A review. Biokhimiya. 69, 725–742.

    Google Scholar 

  50. Vlassov A.V., Florentz C., Helm M., Naumov V.A., Buneva V.N., Nevinsky G.A., Giege R. 1998. Catalytic antibodies from human serum with RNase activity. New tools for investigation of RNA structure. Nucleic Acids Res. 26, 5243–5250.

    Article  PubMed  CAS  Google Scholar 

  51. Ivanen D.R., Kulminskaya A.A., Shabalin K.A., Isaeva-Ivanova L.V., Ershova N.A., Saveliev A.N., Nevinsky G.A., Neustroev K.N. 2004. Catalytic properties of IgMs with amylolytic activity isolated from patients with multiple sclerosis. Med. Sci. Monit. 10, BR273–BR280.

    PubMed  CAS  Google Scholar 

  52. Keinan E. 2005. Catalytic Antibodies. Weinheim: VCH-Wiley.

    Google Scholar 

  53. Shoenfeld Y., Teplizki H.A., Mendlovic S., Blank M., Mozes E., Isenberg D.A. 1989. The role of the human anti-DNA idiotype 16/6 in autoimmunity. Clin. Immunol. Immunopathol. 51, 313–325.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. S. Odintsova, A. G. Baranovskii, V. N. Buneva & G. A. Nevinsky

  2. Institute of Clinical Immunology, Rostov State Medical University, Rostov-on-Don, 344022, Russia

    M. A. Kharitonova & L. P. Sizyakina

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  1. E. S. Odintsova
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  2. M. A. Kharitonova
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  3. A. G. Baranovskii
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  4. L. P. Sizyakina
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  5. V. N. Buneva
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  6. G. A. Nevinsky
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Original Russian Text © E.S. Odintsova, M.A. Kharitonova, A.G. Baranovskii, L.P. Sizyakina, V.N. Buneva, G.A. Nevinsky, 2006, published in Molekulyarnaya Biologiya, 2006, Vol. 40, No. 5, pp. 857–864.

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Odintsova, E.S., Kharitonova, M.A., Baranovskii, A.G. et al. DNA-hydrolyzing IgG antibodies from the blood of patients with acquired immune deficiency syndrome. Mol Biol 40, 770–777 (2006). https://doi.org/10.1134/S0026893306050128

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