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Compatible Solutes Accumulated by Glutamicibacter sp. Strain SMB32 in Response to Abiotic Environmental Factors

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Abstract—

Proton magnetic resonance spectroscopy was used for investigation of the pool of compatible solutes accumulated in the cells of Glutamicibacter sp. strain SMB32 in response to abiotic environmental factors. The original habitat of the strain was anthropogenically salinated soil at the Verkhnekamsk deposit of potassium and magnesium salts (Perm krai, Russia). The strain grew within the temperature range from 5 to 35°C. At 5 and 32°C, the intracellular content of trehalose in the cells of Glutamicibacter sp. SMB32 was significantly higher than at 25°C. Glutamicibacter sp. SMB32 was able to grow both in the absence of NaCl and at its concentrations up to 11%. Glutamate predominated in the cells grown without NaCl. At high salinity (8% NaCl), predominant compounds in the studied strain cells were trehalose, proline, glutamine, and glutamate. Increasing salinity of the growth medium resulted in higher levels of intracellular proline. This is the first report of ability of a Glutamicibacter strain to synthesize mannitol; its accumulation was found to depend on the aeration mode. Thus, Glutamicibacter sp. strain SMB32 possesses high metabolic plasticity and is able to adapt to the action of unfavorable physicochemical factors.

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Funding

This study was carried out within the framework of the State Assignment АААА-А19-119112290008-4.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia

    L. N. Anan’ina, E. A. Shestakova, A. A. Pyankova & E. G. Plotnikova

  2. Institute of Technical Chemistry, Ural Branch, Russian Academy of Sciences, 614013, Perm, Russia

    A. A. Gorbunov

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  1. L. N. Anan’ina
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  2. A. A. Gorbunov
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Correspondence to L. N. Anan’ina.

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Translated by E. Babchenko

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Anan’ina, L.N., Gorbunov, A.A., Shestakova, E.A. et al. Compatible Solutes Accumulated by Glutamicibacter sp. Strain SMB32 in Response to Abiotic Environmental Factors. Microbiology 92, 650–657 (2023). https://doi.org/10.1134/S0026261723601677

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