Abstract
A strain of aerobic anoxygenic phototrophic bacteria (AAPB) isolated from the surface of a cyanobacterial mat of an Eastern Siberian thermal spring (40°C) and designated Se-4 was identified as Porphyrobacter donghaensis according to its 16S rRNA gene sequence. A DNA–DNA hybridization level of 95% was determined between strain Se-4 and the type strain of this species, SW-132T. The isolate was an obligate aerobe, forming orange round colonies on solid media, which turn red in the course of growth. The cells were motile rods capable of branching. The cells divided by uniform fission by constriction. Optimal growth was observed at pH 7.5 and NaCl concentrations from 0 to 1 g/L. The pigments present were carotenoids and bacteriochlorophyll a. Another Porphyrobacter donghaensis strain, Noj-1, isolated from a purple mat developing on the surface of a coastal set-up in a steppe weakly mineralized (1.5 g/L) soda lake Nozhii (Eastern Siberia) possessed similar characteristics. Thus, the AAPB species Porphyrobacter donghaensis was shown to occur, apart from marine environments (its known habitat), also in weakly mineralized soda lakes and freshwater thermal springs. Description of the species Porphyrobacter donghaensis was amended.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Carret, R.H. and Nason, A., Further purification and properties of Neurospora nitrate reductase, J. Biol. Chem., 1969, vol. 244, pp. 2870–2882.
Dawson, R.M.C., Elliott, D.C., Elliott, W.H., and Jones, K.M., Handbook on Biochemistry, Oxford: Oxford. Univ. Press, 1986.
De Ley, J., Cattoir, H., and Reynaerts, A., The quantitative measurements of DNA hybridizaition from renaturation rates, Eur. J. Biochem., 1970, vol. 12, pp. 133–140.
Fuerst, J.A., Hawkins, J.A., Holmes, A., Sly, L.I., Moore, C.J., and Stackebrandt, E., Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyllsynthesizing budding bacterium from fresh water, Int. J. Syst. Bacteriol., 1993, vol. 43, pp. 125–134.
Furuhata, K., Edagawa, A., Miyamoto, H., Kawakami, Y., and Fukuyama, M., Porphyrobacter colymbi sp. nov. isolated from swimming pool water in Tokyo, Japan, J. Gen. Appl. Microbiol., 2013, vol. 59, pp. 245–250.
Hanada, S., Kawase, Y., Hiraishi, A., Takaichi, S., Matsuura, K., Shimada, K., and Nagashima, K.V.P., Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring, Int. J. Syst. Bacteriol., 1997, vol. 47, pp. 408–413.
Hiraishi, A., Yonemitsu, Y., Matsushita, M., Shin, Y.K., Kuraishi, H., and Kawahara, K., Characterization of Porphyrobacter sanguineus sp. nov., an aerobic bacteriochlorophyll- containing bacterium capable of degrading biphenyl and dibenzofuran, Arch. Microbiol., 2002, vol. 178, pp. 45–52.
Marmur, J., A procedure for the isolation of deoxyribonucleic acid from microorganisms, J. Mol. Biol., 1961, vol. 3, pp. 203–218.
Owen, R.J., Hill, L.R., and Lapage, S.P., Determination of DNA base composition from melting profiles in dilute duffers, Biopolymers, 1969, vol. 7, pp. 503–516.
Pfennig, N., Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped vitamin B12 requiring member of the family Rhodospirillaceae, Int. J. Syst. Bacteriol., 1978, vol. 28, pp. 283–288.
Rainey, F.A., Silva, J., Nobre, M.F., Silva, M.T., and Da Costa, M.S. Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species, Int. J. Syst. Evol. Microbiol., 2003, vol. 53, pp. 35–41.
Reynolds, E.S., The use of lead citrate at high pH as an electron opaque stain in electron microscopy, J. Cell. Biol., 1963, vol. 17, pp. 208–218.
Reznikov, A.A., Mulikovskaya, E.P., and Sokolov, Yu.I., Metody analiza prirodnykh vod (Methods for Analysis of Natural Water), Moscow: Nedra, 1970.
Takaichi, S. and Shimada, K., Characterization of carotenoids in photosynthetic bacteria, Methods Enzymol., 1992, vol. 213, pp. 374–385.
Takaichi, S., Carotenoids and carotinogenesis in anoxygenic photosynthetic bacteria, in The Photochemistry of Carotenoids, Frank, H.A., Young, A.J., Britton, G., and Cogdell, R.J., Eds., Dordrecht: Kluwer Academic, 1999, vol. 8, pp. 39–69.
Tsaplina, I.A., Osipov, G.A., Bogdanova, T.I., Nedoresova, T.O., and Karavaiko, G.I., Fatty acid composition of lipids in thermoacidophilic bacteria of the genus Sulfobacillus, Microbiology (Moscow), 1994, vol. 63, no. 5, pp. 459–464.
Van de Peer, Y. and De Wachter, R., TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, Comput. Applic. Biosci., 1994. 10, pp. 569–570.
Yoon, J.H., Kang, S.J., Lee, M.H., Oh, H.W., and Oh, T.K., Porphyrobacter dokdonensis sp. nov., isolated from sea water, Int. J. Syst. Evol. Microbiol., 2006, vol. 56, pp. 1079–1083.
Yoon, J.H., Lee, M.H., and Oh, T.K., Porphyrobacter donghaensis sp. nov., isolated from sea water of the East Sea in Korea, Int. J. Syst. Evol. Microbiol., 2004, vol. 54, pp. 2231–2235.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.N. Nuyanzina-Boldareva, V.N. Akimov, S. Takaichi, V.M. Gorlenko, 2016, published in Mikrobiologiya, 2016, Vol. 85, No. 1, pp. 56–65.
Rights and permissions
About this article
Cite this article
Nuyanzina-Boldareva, E.N., Akimov, V.N., Takaichi, S. et al. New strains of an aerobic anoxygenic phototrophic bacterium Porphyrobacter donghaensis isolated from a Siberian thermal spring and a weakly mineralized lake. Microbiology 85, 77–86 (2016). https://doi.org/10.1134/S0026261716010070
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261716010070