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Application of ribulose-1,5-bisphosphate carboxylase/oxygenase genes as molecular markers for assessment of the diversity of autotrophic microbial communities inhabiting the upper sediment horizons of the saline and soda lakes of the Kulunda Steppe

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Abstract

The genes encoding the key metabolic reactions are often used as functional markers for phylogenetic analysis and microbial ecology studies. The composition and structure of the genes encoding ribulose-1,5-bisphosphate carboxylase (RuBisCO) of various photoautotrophic bacteria, representatives of the order Chromatiales, including collection strains and the strains isolated from saline and soda lakes, were studied in detail. The green-like form I RuBisCO was detected in the majority of the studied strains. In some strains, the genes encoding both form I and form II RuBisCO were present, which has not been previously known for the representatives of this group of bacteria. Moreover, RuBisCO genes were used as functional markers to investigate the autotrophic microbial community inhabiting the upper horizons of bottom sediments of two saline soda lakes and two hypersaline neutral lakes of the Kulunda Steppe. In general, the diversity of autotrophic bacteria in the studied sediment horizons was low. In soda lakes, haloalkaliphilic cyanobacteria and sulfuroxidizing bacteria (SOB) of the genus Halorhodospira were predominant. In saline lakes, halophilic chemoautotrophic SOB Halothiobacillus and Thioalkalivibrio were found, as well as photoautotrophic bacteria of the genus Ectothiorhodosinus and cyanobacteria. Many phylotypes remained unidentified, which indicates the presence of groups of microorganisms with an unknown type of metabolism.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    T. P. Tourova, O. L. Kovaleva, I. A. Bryantseva, V. M. Gorlenko & D. Yu. Sorokin

  2. Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia

    B. K. Bumazhkin, E. O. Patutina & B. B. Kuznetsov

  3. Department of Biotechnology, TU Delft, Delft, The Netherlands

    D. Yu. Sorokin

Authors
  1. T. P. Tourova
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  2. O. L. Kovaleva
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  3. B. K. Bumazhkin
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  4. E. O. Patutina
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  5. B. B. Kuznetsov
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  6. I. A. Bryantseva
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  7. V. M. Gorlenko
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  8. D. Yu. Sorokin
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Correspondence to T. P. Tourova.

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Original Russian Text © T.P. Tourova, O.L. Kovaleva, B.K. Bumazhkin, E.O. Patutina, B.B. Kuznetsov, I.A. Bryantseva, V.M. Gorlenko, D.Yu. Sorokin, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 6, pp. 803–817.

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Tourova, T.P., Kovaleva, O.L., Bumazhkin, B.K. et al. Application of ribulose-1,5-bisphosphate carboxylase/oxygenase genes as molecular markers for assessment of the diversity of autotrophic microbial communities inhabiting the upper sediment horizons of the saline and soda lakes of the Kulunda Steppe. Microbiology 80, 812–825 (2011). https://doi.org/10.1134/S0026261711060221

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