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Molecular and xylanolytic variation identified among strains of Pyrenophora graminea

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Abstract

The inter-retrotransposon amplified polymorphism (IRAP) was used to confirm the genetic variation among 22 strains of Pyrenophora graminea differing in their xylanase production. A total of 162 bands were scored of which 151 (93.21%) were polymorphic. The molecular parameter used showed that P. graminea strains reside in four phylogenetic groups. There was observed the resolution between clustering strains and their xylanase production. Hence, the described approach presented here constitutes no prior assumption about the characterization of P. graminea strains differing in xylanase production.

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References

  1. Wong, K.K.Y., James, C.S., and Campion, S.H., Xylanase Pre and Post-Treatments of Bleached Pulps Decrease Absorption Coefficient, J. Pulp Pap. Sci., 2000, vol. 26, pp. 377–383.

    CAS  Google Scholar 

  2. Loera, O. and Cordova, J., Improvement of Xylanse Production by Parasexual Cross between Aspergillus niger Strains, Braz. Arch. Biol. Technol., 2003, vol. 46, pp. 177–181.

    Article  CAS  Google Scholar 

  3. Guimaraes, L.H.S., Nogueira, P.S., Michelin, M., Rizzatti, A.C.S., Sandrim, V.C., Zanoela, F.F., Aquino, A.C.M.M., Junior A.B., and Polizeli, M.L.T.M., Screening of Filamentous Fungi for Production of Enzymes of Biotechnological Interest, Braz. J. Microbiol., 2006, vol. 37, pp. 474–480.

    Article  CAS  Google Scholar 

  4. Singh, S., Pillay, B., and Prior, B.A., Thermal Stability of β-Xylanase Produced by Different Thermomyces lanuginosus Strains, Enzyme Microb. Technol., vol. 26, pp. 502–208.

  5. Kalendar, R., Grob, T., Regina, M., Suoniem, A., and Schulman, A., IRAP and REMAP: Two New Retrotransposon-Based DNA Fingerprinting Techniques, Theor. Appl. Genet., 1999, vol. 98, pp. 704–711.

    Article  CAS  Google Scholar 

  6. Pasquali, M., Dematheis, F., Lodovica Gullino, M., and Garibaldi, A., Identification of Race1 of Fusarium oxysporum f. sp. lactucae on Lettuce by Inter-Retrotransposon Sequence-Characterized Amplified Region Technique, Phytopathol., 2007, vol. 97, pp. 987–996.

    Article  CAS  Google Scholar 

  7. Kalendar, R., Tanskanen, J., Immonen, S., Nevo, E., and Schulman, A.H., Genome Evolution of Wild Barley (Hordeum spontaneum) by BARE-1 Retrotransposon Dynamics in Response to Sharp Microclimatic Divergence, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 6603–6607.

    Article  PubMed  CAS  Google Scholar 

  8. Vicient, C.M. and Schulman, A.H. Copia-Like Retrotransposons in the rice Genome: Few and Assorted, Genome Lett., 2002. vol. 1, pp. 35–47.

    Article  CAS  Google Scholar 

  9. Arabi, M.I.E., MirAli, N., Jawhar, M., and Al-Safadi, B., Differentiation of Drechslera graminea Isolates by Cultural Characters and SDS-PAGE, J. Plant Pathol., 2002, vol. 84, pp. 153–156.

    CAS  Google Scholar 

  10. Arabi, M.I.E., Jawhar, M., and MirAli, M., Polypeptide Patterns of Syrian Isolates of Pyrenophora graminea, J. Plant Pathol., 2006, vol. 88, pp. 129–132.

    Google Scholar 

  11. Bailey, M.J., Bailey, P., and Poutanen, R., Interlaboratory Testing of Methods for Assay of Xylanase Activity, J. Biotechnol., 1992, vol. 23, pp. 257–270.

    Article  CAS  Google Scholar 

  12. Miller, G.L., Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugars, Ann. Chem., 1959, vol. 31, pp. 426–428.

    Article  CAS  Google Scholar 

  13. Anonymous, STAT-ITCF, Programme, MICROSTA, ECOSOFT, 2nd Ver., Institut Technique des Cereals et des Fourrages, Paris, France, 1988.

  14. Leach, J., Finklstein, D.B., and Ramosek, J.A., Rapid Miniprep of DNA from Filamentous Fungi, Fungal Genet. Newsl., 1986, vol. 33, pp. 32–33.

    Google Scholar 

  15. Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variation in Terms of Restricting Endonucleases, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 5269–5273.

    Article  PubMed  CAS  Google Scholar 

  16. Statsoft, STATISTICA, Data Analysis Software System, 6th ver., www.Statsoft.com. 2003.

  17. Nishimura, M., Hayashi, N., Jwa, N.S., Lau, G.W., and Hamer, J.E., Insertion of the LINE Retrotransposon MGL Causes a Conidiophore Pattern Mutation in Magnaporthe grisea, Mol. Plant-Microb. Interact., 2000, vol.13, pp. 892–894.

    Article  CAS  Google Scholar 

  18. Jawhar, M., Sangwan, R.S., and Arabi, M.I.E., Identification of Drechslera graminea Isolates by Cultural Characters and RAPD Analysis, Cereal Res. Comm., 2000, vol. 28, pp. 89–93.

    Google Scholar 

  19. Farrell, R.L., Biely, P., and McKay, D.L. Production of Hemicellulase by the Fungus Penicillium kloeckeri, in Biotechnology in the Pulp and Paper Industry, Proc. 6th Int. Conf. Biotechnol. Pulp Paper Ind., Srebotnik, E. and Messner, K., Eds., Facultas-universitatsverlag, Vienna, 1996, pp. 485–489.

    Google Scholar 

  20. Palma, M.B., Milagres, A.M.F., Prata, A.M.R., and Manchilha, I.M., Influence of Aeration and Agitation Rate on the Xylanase Activity from Pencillium janthinellum, Process Biochem., 1996. vol. 31, pp. 141–145.

    Article  CAS  Google Scholar 

  21. Ahmed, S., UI-Ain, Q., Aslam, N., Naeem, S., UI-Rahman, S., and Jamil, A., Induction of Xylanase and Cellulose Genes from Trichoderma harzianum with Different Carbon Sources, Pak. J. Biol. Sci., 2003, vol. 22, pp. 1912–1916.

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus, Syria

    Y. Bakri, M. Jawhar & M. I. E. Arabi

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  1. Y. Bakri
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  2. M. Jawhar
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  3. M. I. E. Arabi
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Correspondence to M. I. E. Arabi.

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Bakri, Y., Jawhar, M. & Arabi, M.I.E. Molecular and xylanolytic variation identified among strains of Pyrenophora graminea . Microbiology 80, 391–394 (2011). https://doi.org/10.1134/S0026261711030039

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