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Rubribacterium polymorphum gen. nov., sp. nov., a novel alkaliphilic nonsulfur purple bacterium from an Eastern Siberian soda lake

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Abstract

An alkaliphilic nonsulfur purple bacterial (NPB) strain “Green” was isolated from sediments of the littoral zone of the soda lake (mineralization 22 g/l, pH 9.5) in the Barguzin River valley (Eastern Siberia). The cells of the new isolate are ovoid or polymorphic at latter stages. The photosynthetic membrane structures are of vesicular type. Bacteriochlorophyll a and carotenoids of both spheroidene and spirilloxanthin type are the photosynthetic pigments. Two light-harvesting systems (LH1 and LH2) are present. The new isolate is a photoheterotroph and a facultative aerobe. It grows well in the dark on organic substrates; anaerobic phototrophic growth is poor. The isolate is alkaliphilic with pH optimum of 8.5–9.5. The most abundant cell growth occurred at 5–40 g/l NaCl (optimum at 10 g/l) and 30 °C. The DNA G+C base content was 69.9 mol %. Analysis of 16S rRNA gene sequences revealed a 10% difference with the most closely related NPB (Rhodobacter species). Rubrimonas cliftoensis, a bacteriochlorophyll a-containing bacterium, is the closest relative (93.3% similarity). It is proposed that strain “Green” should be placed in the new genus and new species Rubribacterium polymorphum gen. nov., sp. nov. GenBank accession number: 16S rRNA-EU857676.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya, 7, k. 1, 117312, Moscow, Russia

    E. N. Boldareva, T. P. Tourova & V. M. Gorlenko

  2. Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    A. A. Moskalenko & Z. K. Makhneva

  3. Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya, 7, k. 1, Moscow, 117312, Russia

    T. V. Kolganova

Authors
  1. E. N. Boldareva
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  2. A. A. Moskalenko
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  3. Z. K. Makhneva
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  4. T. P. Tourova
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  5. T. V. Kolganova
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  6. V. M. Gorlenko
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Correspondence to V. M. Gorlenko.

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Boldareva, E.N., Moskalenko, A.A., Makhneva, Z.K. et al. Rubribacterium polymorphum gen. nov., sp. nov., a novel alkaliphilic nonsulfur purple bacterium from an Eastern Siberian soda lake. Microbiology 78, 732–740 (2009). https://doi.org/10.1134/S0026261709060101

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  • DOI: https://doi.org/10.1134/S0026261709060101

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