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Antioxidant systems of moderately thermophilic methanotrophs Methylocaldum szegediense and Methylococcus capsulatus

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Abstract

Moderately thermophilic methanotrophs Methylocaldum szegediense O-12 and Methylococcus capsulatus Bath exhibit activities of antioxidant protection enzymes: glutathione peroxidase, superoxide dismutase, and cytochrome c peroxidase. The cells of methanotrophs grown at optimal temperatures (57 or 45°C, respectively) produce reactive oxygen species more actively than those grown at suboptimal temperatures, and exhibit higher activities of the membrane-associated cytochrome c peroxidase. Glutathione, glutathione peroxidase, and glucose-6-phosphate dehydrogenase levels in Md. szegediense O-12 increased in response to lowering of the cultivation temperature. By contrast, glutathione accumulation in cells of Mc. capsulatus Bath and the activity of glutathione peroxidase at a suboptimal temperature (29°C) were lower than at the optimal one. The role of the multilevel system of antioxidant protection in the adaptation of methanotrophs to temperature fluctuations is discussed.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia

    K. A. Medvedkova, V. N. Khmelenina, N. E. Suzina & Yu. A. Trotsenko

  2. Pushchino State University, Puschino, Russia

    Yu. A. Trotsenko

Authors
  1. K. A. Medvedkova
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  2. V. N. Khmelenina
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  3. N. E. Suzina
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  4. Yu. A. Trotsenko
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Correspondence to Yu. A. Trotsenko.

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Original Russian Text © K.A. Medvedkova, V.N. Khmelenina, N.E. Suzina, Yu.A. Trotsenko, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 6, pp. 723–730.

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Medvedkova, K.A., Khmelenina, V.N., Suzina, N.E. et al. Antioxidant systems of moderately thermophilic methanotrophs Methylocaldum szegediense and Methylococcus capsulatus . Microbiology 78, 670–677 (2009). https://doi.org/10.1134/S0026261709060022

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  • DOI: https://doi.org/10.1134/S0026261709060022

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