Abstract
The effect of kanamycin on the electrophysical parameters of cell suspensions of Escherichia coli K-12 and pMMB33 was investigated. Incubation of the sensitive K-12 strain with kanamycin resulted in significant changes in the orientation spectra (OS) of the cell suspensions; these changes were not revealed in the case of the resistant pMMB33 strain. In the case of the sensitive K-12 strain incubated with different kanamycin concentrations, changes in the OS of the cell suspensions occurred within the 10–1000 kHz frequency range of the orienting electrical field. The most pronounced change in the electrooptical signal was observed at 10 μg/ml of kanamycin. Control experiments were carried out by standard plating on nutrient media. Thus, the OS changes of suspensions in the presence of antibiotics may be used as a test for microbial resistance to such antibiotics.
Similar content being viewed by others
References
Lanchini, D. and Parenti, F., Antibiotiki (Antibiotics), Moscow: Mir, 1985.
Gulii, O.I., Markina, L.N., Ignatov, O.V., Shchegolev, S.Yu., Bunin, V.D., and Ignatov, V.V., The Effect of Ampicillin on the Electrophysical Properties of Escherichia coli Cells, Mikrobiologiya, 2005, vol. 74, no. 1, pp. 126–131 [Microbiology (Engl. Transl.), vol. 74, no. 1, pp. 111–115].
Brian, L.E., Bakterial’naya rezistentnost’ i chuvstvitel’nost’ k khimiopreparatam (Bacterial Resistence and Sensitivity to Chemical Preparations), Moscow: Meditsina, 1984.
Sazykin, Yu.O. and Navashin, P.S., Antibiotics and Bacterial Cell Envelope, Itogi nauki i tekhniki. VINITI. Ser. Biotekhnol., Moscow, 1991, vol. 31, pp. 1–187.
Miroshnikov, A.I., Fomchenkov, V.M., and Ivanov, A.Yu., Elektofizicheskii analiz i razdelenie kletok (Electrophysical Analysis and Separation of Cells), Moscow: Nauka, 1986.
Ignatov, O.V., Guliy, O.I., Shchyogolev, S.Yu., Bunin, V.D., and Ignatov, V.V., Effect of p-Nitrophenol Metabolites on Microbial-Cell Electro-Optical Characteristics, FEMS Microbiol. Letts., 2002, vol. 214, pp. 81–86.
Bunin, V.D. and Voloshin, A.G., Determination of Cell Structures, Electrophysical Parameters, and Cell Population Heterogeneity, J. Colloid Interface Sci., 1996, vol. 180, pp. 122–126.
Bunin, V.D., Voloshin, A.G., Bunin, Z.F., and Shmelev, V.A., Electrophysical Monitoring of Culture Process of Recombinant Escherichia coli Strains, Biotechnol. Bioeng., 1996, vol. 51, pp. 720–724.
Labinskaya, A.S., Mikrobiologiya s tekhnikoi mikrobiologicheskikh issledovanii (Microbiology and Techniques for Microbiological Investigation), Moscow: Meditsina, 1978.
Manual of Methods for General Bacteriology, Gerhardt, P. et al., Eds., Washington: Amer. Soc. Microbiol., 1981 [Russ. Transl. Moscow: Mir, 1984, vol. 1, pp. 458–464].
Spirin, A.S., Molekulyarnaya biologiya. Struktura ribosomy i biosintez belka (Molecular Biology. Ribosome Structure and Protein Synthesis), Moscow: Vysshaya shkola, 1986.
Kudlai, D.G., Chubukov, V., and Oganesyan, M., Genetika lekarstvennoi ustoichivosti bakterii (Genetics of Bacterial Drug Resistance), Moscow: Meditsina, 1972.
Egorov, N.S., Osnovy Ucheniya ob Antibiotikakh (Basic Principles of the Theory of Antibiotics), Moscow: Vysshaya Shkola, 1986.
Galindo, E., Bautista, D., Garcia, J.L., and Quintero, R., Microbial Sensor for Penicillins Using a Recombinant Strain of Escherichia coli, Enz. Microb. Technol., 1990, vol. 12, pp. 642–646.
Galindo, E., Lagunas, F., Osuna, J., Soberon, X., and Garcia, J.L., A Microbial Biosensor for 6-Aminopenicillanic Acid, Enz. Microb. Technol., 1998, vol. 23, pp. 331–334.
Fleschin, S., Bala, C., Bunaciu, A.A., Panait, A., and Aboulenein, H.Y., Enalapril Microbial Biosensor, Prepar. Biochem. Biotechnol., 1998, vol. 28, pp. 261–269.
Cavalieri, S.J., Biehle, J.R., and Sanders, W.E., Jr., Synergistic Activities of Clarithromycin and Antituberculous Drugs Against Multidrug-Resistant Mycobacterium tuberculosis, Antimicrob. Agents Chemother., 1995, vol. 39, no. 7, pp. 1542–1545.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.I. Guliy, L.N. Markina, V.D. Bunin, V.V. Ignatov, O.V. Ignatov, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 3, pp. 380–385.
Rights and permissions
About this article
Cite this article
Guliy, O.I., Markina, L.N., Bunin, V.D. et al. Electrooptical parameters of kanamycin-treated E. coli cell suspensions. Microbiology 77, 334–338 (2008). https://doi.org/10.1134/S0026261708030132
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261708030132