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Involvement of Acetylcholine and Na+,K+-ATPase in the Regulation of Skeletal Muscle Growth in a Chicken Embryo

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Abstract

Recently, much attention has been paid to the discussion of the trophic function of the nervous system and its involvement in triggering the signaling cascades that regulate cellular metabolism. The role of acetylcholine and Na+,K+-ATPase in the regulation of skeletal muscle growth was evaluated in 10–12-day-old chicken embryos under conditions of organotypic tissue culture. Acetylcholine had a maximum trophotropic effect at a concentration 10–8 M. An inhibitory analysis proved the involvement of the nicotinic acetylcholine receptor (nAChR) in this effect. Ouabain regulated the growth of skeletal muscle explants in a dose-dependent manner. At concentrations comparable to the physiological, it stimulated the growth of experimental explants by 33% compared to the control value. Ouabain was found to have myotoxic properties in the concentration range of 10–6–10–4 M. Acetylcholine abolished the myotoxic effect of ouabain (10–6 M) both directly, acting on Na+,K+-ATPase, and via the nAChR.

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Funding

The work was supported by the State Program 47 SP, Scientific and Technological Development of the Russian Federation (2019–2030); Theme 0134-2019-0001.

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Authors and Affiliations

  1. Pavlov First St. Petersburg State Medical University, St. Petersburg, Russia

    E. V. Lopatina, A. V. Gavrichenko & N. A. Pasatetskaya

  2. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    E. V. Lopatina

Authors
  1. E. V. Lopatina
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  2. A. V. Gavrichenko
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  3. N. A. Pasatetskaya
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Contributions

All authors equally contributed to the concept, experimental design, data collection and processing, as well as manuscript preparation. All authors read and approved the final article’s version before submission.

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Correspondence to E. V. Lopatina.

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COMPLIANCE WITH ETHICAL STANDARDS

All the animal-involving experimental procedures complied with ethical standards approved by the legal acts of the Russian Federation, the principles of the Basel Declaration, and the recommendations of the Bioethics Committee at the Pavlov First St. Petersburg State Medical University, St. Petersburg, Russia.

CONFLICT OF INTEREST

The authors declare that they have no conflict of interest.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 2, pp. 229–240https://doi.org/10.31857/S0869813923020073.

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Lopatina, E.V., Gavrichenko, A.V. & Pasatetskaya, N.A. Involvement of Acetylcholine and Na+,K+-ATPase in the Regulation of Skeletal Muscle Growth in a Chicken Embryo. J Evol Biochem Phys 59, 285–292 (2023). https://doi.org/10.1134/S0022093023010234

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