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Glutathione antioxidant complex and carotenoid composition in tissues of the bivalve mollusk Anadara kagoshimensis (Tokunaga, 1906)

  • Comparative and Ontogenic Biochemistry
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Abstract

The correlation of the state of glutathione complex composed of reduced glutathione (GSH), glutathione reductase (GR) activity and glutathione peroxidase (GP) and the qualitative composition of carotenoids was investigated in the bivalve mollusk Anadara kagoshimensis (Tokunaga, 1906). Using high-performance liquid chromatography, UV-Vis and mass spectra, 7 types of carotenoids (trans- and cis-pectenolon, alloxanthine, pectenol A, β-carotene, zeaxanthin and diatoxanthin) were identified in tissues of this species and their quantitative ratio was determined. A positive correlation (R 2 > 0.9) was established between GSH and most carotenoid levels. A negative correlation was found for the GR–carotenoids (R 2 > 0.75) and GP–pectenol A (R 2 > 0.988) systems. The cause-and-effect relations of these regularities are discussed.

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References

  1. Sies, H., Oxidative stress: oxidants and antioxi dants, Exp. Physiol., 1997, vol. 82, pp. 291–295.

    Article  CAS  PubMed  Google Scholar 

  2. Padayatty, S.J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.H., Chen, S., Corpe, C., Dutta, A., Dutta, S.K., and Levine, M., Vitamin C as an antioxidant: evaluation of its role in disease prevention, J. Am. Coll. Nutr., 2003, vol. 22, pp. 18–35.

    Article  CAS  PubMed  Google Scholar 

  3. Traber, M.G. and Atkinson, J., Vitamin E, antioxidant and nothing more, Free Rad. Biol. Med., 2007, vol. 43, pp. 4–15.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Sheehan, D., Mcintosh, J., Power, A., and Fitzpatrick, P.J., Environmental biochemistry, Biochem. Soc. Trans., 1995, vol. 23, pp. 419–422.

    Article  CAS  PubMed  Google Scholar 

  5. Romeo, M. and Gnassia-Barelli, M., Effect of heavy metal on lipid peroxidation in the Mediterranean clam, Ruditapes decussates, Comp. Biochem. Physiol., 1997, vol. 118, pp. 33–37.

    Google Scholar 

  6. Viarengo, A., Canesi, L., Garcia-Martinez, P., Peters, L.D., and Livingstone, D.R., Pro-oxidant processes and antioxidant defense systems in the tissues of the Antarctic scallop (Adamussium colbecki) compared with the Mediterranean scallop (Pecten jacobaeus), Comp. Biochem. Physiol., 1995, vol. 111 B, pp. 119–126.

    Article  Google Scholar 

  7. Cossu, C., Doyotte, A., Babut, M., Exinger, A., and Vasseur, P., Antioxidant biomarkers in freshwater bivalves, Unio tumidus, in response to different contamination profiles of aquatic sediments, Ecotoxicol. Environ. Safety, 2000, vol. 45, pp. 106–121.

    Article  CAS  PubMed  Google Scholar 

  8. Soldatov, A.A., Gostyukhina, O.L., and Golovina, I.V., State of the antioxidant enzyme complex in tissues of the Black Sea mollusc Mytilus galloprovincialis under natural oxidative stress, J. Evolutionary Biochem. Physiol., 2008, vol. 44, pp. 175–182.

    Article  CAS  Google Scholar 

  9. Soldatov, A.A., Andreenko, T.I., Golovina, I.V., and Stolbov, A.Ya., Peculiarities of organization of tissue metabolism in mollusks with different tolerance to external hypoxia, J. Evolutionary Biochem. Physiol., 2010, vol. 46, pp. 341–349.

    Article  CAS  Google Scholar 

  10. Niyogi, S., Biswas, S., Sarker, S., and Datta, A.G., Antioxidant enzymes in brackishwater oyster, Saccostrea cucullata as potential biomarkers of polyaromatic hydrocarbon pollution in Hooghly Estuery (India): seasonality and its consequences, Sci. Total Environ., 2001, vol. 281, pp. 237–246.

    Article  CAS  PubMed  Google Scholar 

  11. Carotenoids. Biosynthesis and Metabolism, Britton, G., Liaaen-Jensen, S., and Pfander, H., Eds., vol. 3, Basel, 1998.

  12. Carotenoids. Natural Functions, Britton,G., Liaaen-Jonsen, S., and Pfander, H., Eds., vol. 4, Basel, 2008.

  13. Shimidzu, N., Goto, M., and Miki, W., Carotenoids as singlet oxygen quenchers in marine organisms, Fisheries Science, 1996, vol. 62, 1996, pp. 134–137.

    Article  CAS  Google Scholar 

  14. Polyakov, N.E. and Leshina, T.V., Some aspects of the reactive ability of carotenoids. Redox processes and complex formation, Usp. Khim., 2006, vol. 75, pp. 1175–1192.

    Article  Google Scholar 

  15. Gostyukhina, O.L., Soldatov, A.A., Borodina, A.V., and Golovina, I.V., Content of carotenoids and the state of tissue antioxidant enzymatic complex in bivalve mollusc Anadara inaequivalvis Br., J. Evol. Biochem. Physiol., 2013, vol. 49, pp. 309–315.

    Article  CAS  Google Scholar 

  16. Soldatov, A.A., Gostyukhina, O.L., Borodina, A.V., and Golovina, I.V., Qualitative composition of carotenoids, catalase and superoxide dismutase activities in tissues of the bivalve mollusc Anadara inaequivalvis (Bruguiere, 1789), J. Evol. Biochem. Physiol., 2013, vol. 49, pp. 389–398.

    Article  CAS  Google Scholar 

  17. Ribera, D., Narbonne, J.F., Daubeze, M., and Michel, X., Characterization, tissue distribution and sexual differences of some parameters related to lipid peroxidation in mussels, Mar. Environ. Res., 1989, vol. 28, pp. 279–283.

    Article  CAS  Google Scholar 

  18. Maoka, T. and Akimoto, N., Natural product chemistry in carotenoid some experimental techniques for structural elucidation and analysis of natural carotenoids, Carotenoid Science (Mini-review), 2008, vol. 13, pp. 10–17.

    Google Scholar 

  19. Repeta, D.J. and Bjornland, T., Preparation of carotenoid standards, in Phytoplankton pigments in oceanography: guidelines to modern methods, UNESCO: Paris, 1997, pp. 239–260.

    Google Scholar 

  20. Karnaukhov, V.N., Biologicheskie funktsii karotinoidov (Biological Functions of Carotenoids), Moscow, 1988.

    Google Scholar 

  21. Pereslegina, I.A., Activity of antioxidant enzymes in saliva of healthy children, Lab Delo, 1989, vol. 11, pp. 20–23.

    Google Scholar 

  22. Putilina, F.E., Measurement of reduced glutathione in tissues, Metody biokhimicheskikh issledovanii (Methods of Biochimical Studies), Leningrad, 1982, pp 183–186.

    Google Scholar 

  23. Terao, J., Antioxidant activity of beta-carotene-related carotenoids in solution, Lipids, 1989, vol. 24, pp. 659–661.

    Article  CAS  PubMed  Google Scholar 

  24. Kulinsky, V.I. and Kolesnichenko, L.S., Biological role of glutathione, Usp. Sovr. Biol., 1990, vol. 110, pp. 20–33.

    Google Scholar 

  25. Menshikova, E.B. and Zenkov, N.K., Antioxidants and inhibitors of radical oxidative processes, Usp. Sovr. Biol., 1993, vol. 113, pp. 442–455.

    Google Scholar 

  26. Woodall, A.A., Britton, G., and Jackson, M.J., Carotenoids and protection of phospholipids in solution or in liposomes against oxidation by peroxyl radicals: relationship between carotenoid structure and protective ability, Biochim. Biophys. Acta, 1997, vol. 1336, pp. 575–586.

    Article  CAS  PubMed  Google Scholar 

  27. Britton, G., Structure and properties of carotenoids in relation to function, J. FASEB, 1995, vol. 9, pp. 1551–1558.

    CAS  Google Scholar 

  28. Fernandes, A.P. and Holmgren, A., Glutaredoxins: glutathione-dependent redox enzymes with functions far beyond a simple thioredoxin backup system, Antioxid. Redox Signal., 2004, vol. 6, pp. 63–74.

    Article  CAS  PubMed  Google Scholar 

  29. Yeum, K.-J., Aldini, G., Russell, R.M., and Krinsky, N.I., Carotenoids. Antioxidant/Pro-oxidant Actions of Carotenoids, Britton, G., Liaaen-Jensen, S., and Pfander, H., Eds., vol. 5, Basel, 2009, pp. 235–262.

    Google Scholar 

  30. Maoka, T., Hashimoto, K., Akimoto, N., and Fujiwara, Y., Structures of five new carotenoids from the oyster Crassostrea gigas, J. Nat. Prod., 2001, vol. 64, pp. 578–581.

    Article  CAS  PubMed  Google Scholar 

  31. Martin, H.D., Ruck, C., Schmidt, M., Sell, S., Beutner, S., Mayer, B., and Walsh, R., Chemistry of carotenoid oxidation and free radical reactions, Pure Appl. Chem., 1999, vol. 71, pp. 2253–2262.

    Article  CAS  Google Scholar 

  32. El-Agamey, A., Lowe, G.M., McGarvey, D.J., Mortensen, A., Phillip, D.M., Truscott, T.G., and Young, A.J., Carotenoid radical chemistry and antioxidant/ pro-oxidant properties, Arch. Biochem. Biophys., 2004, vol. 430, pp. 37–48.

    Article  CAS  PubMed  Google Scholar 

  33. Hill, T.J., Land, E.J., McGarvey, D.J., Schalch, W., Tinkler, J.H., and Truscott, T.G., Interactions between carotenoids and the ccl3o2-center-dot radical, Am. Chem. Soc., 1995, vol. 117, pp. 8322–8326.

    Article  CAS  Google Scholar 

  34. Woodall, A.A., Britton, G., and Jackson, M.J., Carotenoids and protection of phospholipids in solution or in liposomes against oxidation by peroxyl radicals: relationship between carotenoid structure and protective ability, Biochim. Biophys. Acta, 1997, vol. 1336, pp. 575–586.

    Article  CAS  PubMed  Google Scholar 

  35. Bhabak, K.P. and Mugesh, G., Functional mimics of glutathione peroxidase: bioinspired synthetic antioxidants, Acc. Chem. Res., 2010, vol. 43, pp. 1408–1419

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences, Sevastopol, Russia

    A. A. Soldatov, O. L. Gostyukhina, A. V. Borodina & I. V. Golovina

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  1. A. A. Soldatov
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  2. O. L. Gostyukhina
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  3. A. V. Borodina
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  4. I. V. Golovina
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Correspondence to A. A. Soldatov.

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Original Russian Text © A.A. Soldatov, O.L. Gostyukhina, A.V. Borodina, I.V. Golovina, 2017, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2017, Vol. 53, No. 4, pp. 257—264.

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Soldatov, A.A., Gostyukhina, O.L., Borodina, A.V. et al. Glutathione antioxidant complex and carotenoid composition in tissues of the bivalve mollusk Anadara kagoshimensis (Tokunaga, 1906). J Evol Biochem Phys 53, 289–297 (2017). https://doi.org/10.1134/S0022093017040056

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  • DOI: https://doi.org/10.1134/S0022093017040056

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