Skip to main content
Log in

Effects of barium and ouabain on electrogenesis in various sites of intact and detubulated skeletal muscle fibers of the frog R. temporaria

  • Comparative and Ontogenic Physiology
  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

In loose patch clamp experiments on intact sartorius muscle fibers of the frog Rana temporaria, there have been two types of waveforms of extracellularly recorded action potentials (AP). Responses of the first type (T1AP) consisted of an initial positive phase with a subsequent phase of strong negativity, the latter only in a few cases followed by a weak positive phase. Responses of the second type (T2AP) always had an additional positive phase concluding their waveform. In the detubulated fibers, only T1AP were recorded. Application of Ba2+ (10 μM) to the muscle led to a significant increase in the amplitude of the third T2AP phase whereas the T1AP characteristics of both intact and detubulated muscle preparations remained unchanged. In some of the studied intact fibers, after Ba2+ or ouabain (50 μM) applied, the latest positive signal phase was replaced by a negative phase. The amplitude of this latest negative phase was increased markedly by highfrequency stimulation. Under the simultaneous action of ouabain and Ba2+, there was a summation of their effects. Our results can be tentatively explained by that the T-system of a muscle fiber produces electrical responses substantially differing in their pattern and barium sensitivity from those transmitted across cell membranes. These differences could be resultant from the activity of T-tubular inwardly rectifying potassium channels (Kir) and that of Na,K-ATPase as they both provide absorption of excessive extracellular potassium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kristensen, M., Hansen, T., and Juel, C., Membrane Proteins Involved in Potassium Shift during Muscle Activity and Fatigue, Am. J. Physiol. Regul. Comp. Physiol., 2006, vol. 290, R766–R772.

    Article  CAS  Google Scholar 

  2. Kristensen, M. and Juel, C., Potassium-Transporting Proteins in Skeletal Muscle: Cellular Location and Fibre-Type Differences, Acta Physiol., 2010, vol. 198. pp. 105–123.

    Article  CAS  Google Scholar 

  3. Clausen, T., Na+-K+ Pump Regulation and Skeletal Muscle Contractility, Physiol. Rev., 2003, vol. 83, pp. 1269–1324.

    PubMed  CAS  Google Scholar 

  4. Wallinga, W., Mejer, S.L., Alberink, M.J., Vliek, M., Wienk, E.D., and Ypey, D.L., Modelling Action Potentials and Membrane Currents of Mammalian Skeletal Muscle in Coherence with Potassium Concentration Changes in the TTubular System, Eur. Biophys. J., 1999, vol. 28, pp. 317–329.

    Article  PubMed  CAS  Google Scholar 

  5. Kubasov, I.V. and Dobretsov, M.G., Characteristics of Spreading Action Potentials Recorded in Various Sites of Skeletal Muscle Fibers of the Frog R. temporaria, Zh. Evol. Biokhim. Fiziol., 2001, vol. 47, pp. 414–416.

    Google Scholar 

  6. Wolters, H., Wallinga, W., Ypey, D.L., and Boom, H.B.K., Ionic Current during Action Potential in Mammalian Skeletal Muscle Fibers Analyzed with Loose Patch Clamp, Am. J. Physiol. Cell Physiol., 1994, vol. 267, pp. 1699–1706.

    Google Scholar 

  7. Krolenko, S.A., T-sistema myshechnykh volokon: struktura i funktsiya (T-System in Muscle Fibers: Structure and Function), Leningrad, 1975.

  8. Sheikh, S.M., Skepper, N.J., Chawla, S., Vandenberg, J.I., Elneil, S., and Huang, C.L.H., Normal Conduction of Surface Action Potentials in Detubulated Amphibian Skeletal Muscle Fibre, J. Physiol., 2001, vol. 535, Issue 2, pp. 579–590.

    Article  Google Scholar 

  9. Almers, W., Stanfield, P.R., and Stuhmer, W., Lateral Distribution of Sodium and Potassium Channels in Frog Skeletal Muscle: Measurement with Patch Clamp Technique, J. Physiol., 1983, vol. 336, pp. 261–284.

    PubMed  CAS  Google Scholar 

  10. Brigant, J.L. and Mallart, A., Presynaptic Currents in Mouse Motor Ending, J. Physiol., 1982, vol. 333, pp. 619–636.

    PubMed  CAS  Google Scholar 

  11. Sigworth, F.J., Sakmann, B., and Neher, E., Registratsiya odinochnykh kanalov (Single-Channel Recording), Moscow, 1987.

  12. Mallart, A., A Calcium-Activated Potassium Current in Motor Nerve Terminals of the Mouse, J. Physiol., 1985, vol. 368, pp. 577–591.

    PubMed  CAS  Google Scholar 

  13. Agnew, W.S., Dual Roles for DHP Receptors in Excitation-Contraction Coupling, Nature, 1987, vol. 328, p. 297.

  14. Rios, E. and Pizzaro, G., Voltage Sensor of Excitation-Contraction Coupling in Skeletal Muscle, Physiol. Rev., 1991, vol. 7, pp. 887–890.

    Google Scholar 

  15. Matyushkin, D.P., Funktsional’nye kletochnye vzaimodeistviya v nervno-myshechnom apparate (Functional Cell Interactions in the Nerve-Muscle Apparatus), Leningrad, 1980.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to I. V. Kubasov.

Additional information

Original Russian Text © I.V. Kubasov, M.G. Dobretsov, 2012, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2012, Vol. 48, No. 4, pp. 360–366.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kubasov, I.V., Dobretsov, M.G. Effects of barium and ouabain on electrogenesis in various sites of intact and detubulated skeletal muscle fibers of the frog R. temporaria . J Evol Biochem Phys 48, 422–429 (2012). https://doi.org/10.1134/S0022093012040068

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0022093012040068

Key words

Navigation