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The brain is one of the sources of L-dihydroxyphenylalanine in systemic circulation in fetuses and neonatal rats

  • Comparative and Ontogenic Biochemistry
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Abstract

The performed study was aimed at checking our hypothesis that the developing brain is a source of L-dihydroxyphenylalanine (L-DOPA), a precursor of dopamine in the total circulation system. At the initial stage, the L-DOPA concentration in peripheral blood was analyzed at the 18th and 21st embryonal days (E18 and E21), at the 3rd postnatal day (P3), and at the prepubertal period (P30). The highest L-DOPA concentration was revealed at the perinatal period, while decreased 4–12 times for the first month of life. The subsequent analysis of dynamics of the total blood L-DOPA content showed that maintenance of the constant L-DOPA concentration at the perinatal period on the background of a gradual increase of the blood serum volume is due to a rise of its secretion. At the postnatal period (P3–P30), the blood L-DOPA content increased twice in males, whereas it decreased to the same extent in females. Analysis of the L-DOPA concentration in two most important brain centers, hypothalamus and mesencephalon-rhombencephalon, showed its twofold decrease in hypothalamus during E18–E21 of development; then it slightly increased from E21 to P3 and fell 4–5 times by P30. In mesencephalon-rhombencephalon, the L-DOPA concentration was slightly reduced from E18 to E21 (only in females), while on P3 it returned to the E18 level and decreased 7–9 times by P30. The direct proof for the L-DOPA release from the developing brain into the systemic circulation follows from comparison of the blood L-DOPA concentration in shamoperated and encephalectomized rat fetuses after mechanical destruction of neurons of the two abovementioned most important dopaminergic centers. Thus, encephalectomy led to a twofold reduction of the blood L-DOPA concentration (statistically significant differences were observed only in females). Thus, the work presents evidence that the developing brain is one of L-DOPA sources in the total circulation system in rats during prenatal and early postnatal periods of ontogenesis.

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Authors and Affiliations

  1. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    V. I. Melnikova, A. Ya. Sapronova, E. V. Proshlyakova, S. N. Voronova & M. V. Ugryumov

  2. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia

    A. V. Lavrentyeva

  3. State Medical University of the Russian Federation Ministry of Health, Moscow, Russia

    S. I. Ogurtsov

Authors
  1. V. I. Melnikova
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  2. A. Ya. Sapronova
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  3. A. V. Lavrentyeva
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  4. E. V. Proshlyakova
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  5. S. N. Voronova
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  7. M. V. Ugryumov
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Original Russian Text © V.I. Melnikova, A.Ya. Sapronova, A.V. Lavrentyeva, E.V. Proshlyakova, S.N. Voronova, S.I. Ogurtsov, and M.V. Ugryumov, 2006, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2006, Vol. 42, No. 1, pp. 19–25.

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Melnikova, V.I., Sapronova, A.Y., Lavrentyeva, A.V. et al. The brain is one of the sources of L-dihydroxyphenylalanine in systemic circulation in fetuses and neonatal rats. J Evol Biochem Phys 42, 21–29 (2006). https://doi.org/10.1134/S0022093006010030

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  • DOI: https://doi.org/10.1134/S0022093006010030

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