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Characterization of lipodisc nanoparticles containing sensory rhodopsin II and its cognate transducer from Natronomonas pharaonis

  • Cell Biophysics
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Abstract

We describe the preparation and properties of lipodisc nanoparticles–lipid membrane fragments with a diameter of about 10 nm, stabilized by amphiphilic synthetic polymer molecules. We used the lipodisc nanoparticles made of Escherichia coli polar lipids and compared lipodisc nanoparticles that contained the photosensitive protein complex of the sensory rhodopsin with its cognate transducer from the halobacterium Natronomonas pharaonis with empty lipodisc nanoparticles that contained no protein. The lipodisc nanoparticles were characterized by dynamic light scattering, transmission electron microscopy and atomic force microscopy. We found that the diameter of lipodisc nanoparticles was not affected by incorporation of the protein complexes, which makes them a prospective platform for single-molecule studies of membrane proteins.

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Authors and Affiliations

  1. Department of bioengineering, Faculty of Biology, Lomonosov Moscow State University, Moscow, 119234, Russia

    D. V. Bagrov, G. A. Armeev, G. S. Gluhov, T. T. Ismagulova, M. P. Kirpichnikov & K. V. Shaitan

  2. Department of Physics, University of Osnabrück, Osnabrück, 49069, Germany

    N. Voskoboynikova, W. Mosslehy, A. Y. Mulkidjanian & H. -J. Steinhoff

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  1. D. V. Bagrov
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  2. N. Voskoboynikova
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  3. G. A. Armeev
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  4. W. Mosslehy
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  5. G. S. Gluhov
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  6. T. T. Ismagulova
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  7. A. Y. Mulkidjanian
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  8. M. P. Kirpichnikov
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  9. H. -J. Steinhoff
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  10. K. V. Shaitan
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Correspondence to D. V. Bagrov.

Additional information

Original Russian Text © D.V. Bagrov, N. Voskoboynikova, G.A. Armeev, W. Mosslehy, G.S. Gluhov, T.T. Ismagulova, A.Y. Mulkidjanian, M.P. Kirpichnikov, H.-J. Steinhoff, K.V. Shaitan, 2016, published in Biofizika, 2016, Vol. 61, No. 6, pp. 1139–1148.

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Bagrov, D.V., Voskoboynikova, N., Armeev, G.A. et al. Characterization of lipodisc nanoparticles containing sensory rhodopsin II and its cognate transducer from Natronomonas pharaonis . BIOPHYSICS 61, 942–949 (2016). https://doi.org/10.1134/S0006350916060063

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  • DOI: https://doi.org/10.1134/S0006350916060063

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