Abstract
A study was made of the effect of binuclear dinitrosyl iron complexes (DNIC) with glutathione in rats with experimental endometriosis. The latter was induced in an autotransplantation model, where two fragments of endometrium with myometrium (2 × 2 mm) from the left uterine horn were grafted to the inner surface of the anterior abdominal wall. After 4 weeks, the test animals received i.p. injections of 0.5 mL DNIC-glutathione at a dose of 12.5 μmol/kg daily for 12 days. This treatment more than halved the total volume of endometrioid tumors. Remarkably, tumor growths from grafts in control rats were often attended by tumors spontaneously arising nearby or in other locations; no such secondary tumors were observed in DNIC-treated animals. The EPR signal with g av = 2.03 characteristic of protein-bound DNIC with thiol ligands was recorded in liver and endometrioid implants of control as well as treated animals. Activation of ribonucleotide reductase, detected by a doublet EPR signal at g = 2.0 with 2.3-mT hyperfine splitting, was found in small tumors. The beneficial effect of DNIC-glutathione was suggested to be due to DNIC breakdown near the tumors, with release of a large amount of molecular nitric oxide and nitrosonium ions that resulted in selective local cytotoxicity.
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Original Russian Text © L.V. Adamyan, E.N. Burgova, N.A. Tkachev, V.D. Mikoyan, A.A. Stepanyan, M.M. Sonova, A.V. Galkin, A.F. Vanin, 2013, published in Biofizika, 2013, Vol. 58, No. 2, pp. 302–312.
The experimental material reported herein fully corresponds to the original work but the text and data presentation had to be substantially revised for the English version. A.G.
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Adamyan, L.V., Burgova, E.N., Tkachev, N.A. et al. Dinitrosyl iron complexes with glutathione largely relieve rats of experimental endometriosis. BIOPHYSICS 58, 222–227 (2013). https://doi.org/10.1134/S0006350913020036
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DOI: https://doi.org/10.1134/S0006350913020036