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Live-Cell Super-resolution Fluorescence Microscopy

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Abstract

Super-resolution fluorescence microscopy (nanoscopy) enables imaging with a spatial resolution much higher than the diffraction limit of optical microscopy. However, the methods of fluorescence nanoscopy are still poorly suitable for studying living cells. In this review, we describe some examples of live nanoscopy-based discoveries and focus on the development of methods for nanoscopy and specific fluorescent labeling aimed to decrease the damaging effects of light illumination on live samples.

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Abbreviations

3B:

Bayesian analysis of bleaching and blinking

EM-CCD:

electron-multiplying charge-coupled device

FAP:

fluorogen-activating protein

FLINC:

fluorescence fluctuation increase by contact

GFP:

green fluorescent protein

LLSM:

lattice light sheet microscopy

PAINT:

points accumulation for imaging in nanoscale topography

RESOLFT:

reversible saturable/switchable optical linear fluorescence transition

sCMOS:

scientific complementary metal-oxide-semiconductor

SIM:

structured illumination microscopy

SMLM:

single molecule localization microscopy

SOFI:

super-resolution optical fluctuation imaging

SRRF:

super-resolution radial fluctuation

STED:

stimulated emission depletion

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  1. Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    A. S. Mishin & K. A. Lukyanov

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  1. A. S. Mishin
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Russian Text © A. S. Mishin, K. A. Lukyanov, 2019, published in Uspekhi Biologicheskoi Khimii, 2019, Vol. 59, pp. 39–66.

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Mishin, A.S., Lukyanov, K.A. Live-Cell Super-resolution Fluorescence Microscopy. Biochemistry Moscow 84 (Suppl 1), 19–31 (2019). https://doi.org/10.1134/S0006297919140025

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