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Separation and study of the range of plasminogen isoforms in patients with prostate cancer

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Abstract

Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher pI values compared to isoforms found in the control group.

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Abbreviations

DTT:

dithiothreitol

MALDI-TOF:

matrix-assisted laser desorption/ionization time of flight

MASCOT score:

index of reliability

MS:

mass spectrometry

m/z :

mass/charge ratio

PBS:

phosphate buffered saline

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Authors and Affiliations

  1. Institute of Arid Zones, Southern Scientific Center of the Russian Academy of Sciences, pr. Chekhova 41, 344006, Rostov-on-Don, Russia

    E. F. Shin, D. I. Vodolazhsky, A. Yu. Golikov, T. N. Belova, N. V. Boyko, D. V. Zimakov, E. N. Cherkasova, V. A. Tarasov & D. G. Matishov

  2. Rostov State Medical University, Pereulok Nakhichevanskii 29, 344022, Rostov-on-Don, Russia

    M. I. Kogan & M. B. Chibichian

  3. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121, Moscow, Russia

    S. A. Moshkovskii

Authors
  1. E. F. Shin
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  2. D. I. Vodolazhsky
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  3. A. Yu. Golikov
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  4. T. N. Belova
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  5. N. V. Boyko
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  6. D. V. Zimakov
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  7. E. N. Cherkasova
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  8. M. I. Kogan
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  9. M. B. Chibichian
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  10. S. A. Moshkovskii
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  11. V. A. Tarasov
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  12. D. G. Matishov
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Corresponding author

Correspondence to E. F. Shin.

Additional information

Original Russian Text © E. F. Shin, D. I. Vodolazhsky, A. Yu. Golikov, T. N. Belova, N. V. Boyko, D. V. Zimakov, E. N. Cherkasova, M. I. Kogan, M. B. Chibichian, S. A. Moshkovskii, V. A. Tarasov, D. G. Matishov, 2012, published in Biokhimiya, 2012, Vol. 77, No. 9, pp. 1284–1291.

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Shin, E.F., Vodolazhsky, D.I., Golikov, A.Y. et al. Separation and study of the range of plasminogen isoforms in patients with prostate cancer. Biochemistry Moscow 77, 1065–1071 (2012). https://doi.org/10.1134/S0006297912090143

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  • DOI: https://doi.org/10.1134/S0006297912090143

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