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Destabilase-lysozyme of medicinal leech. Multifunctionality of recombinant protein

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Abstract

Preparation and purification of a recombinant protein are described along with characteristics of its specific (for ɛ-(γ-Glu)-Lys and D-dimer substrates) and nonspecific (for L-γ-Glu-pNA) isopeptidase activities; the absence of peptidase function for α-(α-Glu)-Lys substrate is noted. It is shown that the protein exhibits muramidase (cell walls of Micrococcus lysodeikticus) and specific glycosidase activities. The latter was determined towards the fluorogenic substrate 4-methylum-belliferyl-tetra-N-acetyl-β-chitotetraoxide. Antimicrobial activity of recombinant destabilase-lysozyme protein (recDest-Lys) and its 11-membered amphipathic peptide was revealed towards cells of the strict anaerobic Archaean Methanosarcina barkeri, whose cell walls contain no murein. Possible mechanisms of the effect of recDest-Lys on these cells are discussed.

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Abbreviations

CM:

cytoplasmic membrane

CW:

cell wall

(GlcNAc)4-MeU:

4-methylumbelliferryl-tetra-N-acetyl-β-chitotetraoxide

recDest-Lys:

recombinant destabilase-lysozyme

SEM:

scanning electron microscopy

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 119997, Moscow, Russia

    L. L. Zavalova

  2. Research Institute of Physicochemical Medicine, ul. Malaya Pirogovskaya 1a, 119992, Moscow, Russia

    V. N. Lazarev & S. A. Levitsky

  3. Biological Faculty, Lomonosov Moscow State University, 119991, Moscow, Russia

    T. G. Yudina & I. P. Baskova

Authors
  1. L. L. Zavalova
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  2. V. N. Lazarev
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  3. S. A. Levitsky
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  4. T. G. Yudina
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  5. I. P. Baskova
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Corresponding author

Correspondence to I. P. Baskova.

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Original Russian Text © L. L. Zavalova, V. N. Lazarev, S. A. Levitsky, T. G. Yudina, I. P. Baskova, 2010, published in Biokhimiya, 2010, Vol. 75, No. 9, pp. 1314–1324.

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Zavalova, L.L., Lazarev, V.N., Levitsky, S.A. et al. Destabilase-lysozyme of medicinal leech. Multifunctionality of recombinant protein. Biochemistry Moscow 75, 1173–1181 (2010). https://doi.org/10.1134/S0006297910090129

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  • DOI: https://doi.org/10.1134/S0006297910090129

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