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Effect of transient acid stress on the proteome of intestinal probiotic Lactobacillus reuteri

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Abstract

We report the acid tolerance response and changes in the level of protein expression of probiotic Lactobacillus reuteri subjected to transient (1.5 h) acid stress at pH 3.0. Sixteen acid-responsive proteins were identified by peptide mass fingerprinting including members of five broad functional categories: metabolism, transcription/translation, DNA replication/repair, transport and binding proteins, and pH homeostasis and stress responses. This work can provide some new and relevant information on the inducible mechanisms underlying the capacity of probiotic L. reuteri to tolerate acid stress.

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Abbreviations

IEF:

isoelectric focusing

IPG:

immobilized pH gradient

MALDI-TOF-MS:

matrix-assisted laser desorption/ionization time of flight mass spectrometry

MRS:

DeMan-Rogosa-Sharpe medium

PMF:

proton motive force

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Authors and Affiliations

  1. Department of Biotechnology, SongDo Techno Park, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, Korea, 406-840

    KiBeom Lee

  2. Research Institute, AdipoGen, Room #401, Venture Building B, SongDo TechnoPark, 7-50 Songdo-Dong, Yeonsu-Gu, Incheon, Korea, 406-840

    KyungBae Pi

Authors
  1. KiBeom Lee
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  2. KyungBae Pi
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Correspondence to KiBeom Lee.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 4, pp. 558–564.

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Lee, K., Pi, K. Effect of transient acid stress on the proteome of intestinal probiotic Lactobacillus reuteri . Biochemistry Moscow 75, 460–465 (2010). https://doi.org/10.1134/S0006297910040097

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  • DOI: https://doi.org/10.1134/S0006297910040097

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