Skip to main content
SpringerLink
Log in

Interaction of yeast mitochondria with fatty acids and mitochondria-targeted lipophilic cations

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

The effect of fatty acids and mitochondria-targeted lipophilic cations (SkQ1, SkQ3, MitoQ, and C12TPP) on tightly-coupled mitochondria from yeasts Dipodascus (Endomyces) magnusii and Yarrowia lipolytica was investigated. Micromolar concentrations of saturated and unsaturated fatty acids were found to decrease the membrane potential, which was recovered almost totally by ATP and BSA. At low, micromolar concentrations, mitochondria-targeted lipophilic cations are “relatively weak, mild uncouplers”, at higher concentrations they inhibit respiration in state 3, and at much higher concentrations they induce swelling of mitochondria, possibly due to their prooxidant and detergent action. At very low, not uncoupling concentrations, mitochondria-targeted lipophilic cations profoundly promote (potentiate) the uncoupling effect of fatty acids. It is conceivable that the observed uncoupling effect of lipophilic cations can be, at least partially, due to their interactions with the endogenous pool of fatty acids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

CTAB:

cetyltrimethylammonium bromide

C12TPP:

dodecyltriphenylphosphonium

MitoQ:

10-(6′-ubiquinonyl)decyltriphenylphosphonium

ROS:

reactive oxygen species

SkQ1:

10-(6′-plastoquinonyl)decyltriphenylphosphonium

SkQ3:

10-(5-methylplastoquinonyl)decyltriphenylphosphonium

Δψ:

mitochondrial transmembrane potential

References

  1. Zorov, D. B., Isaev, N. K., Plotnikov, E. Y., Zorova, L. D., Stelmashook, E. V., Vasileva, A. K., Arkhangelskaya, A. A., and Khryapenkova, T. G. (2007) Biochemistry (Moscow), 72, 1115–1126.

    Article  CAS  Google Scholar 

  2. Harman, D. (1956) Gerontology, 11, 298–300.

    CAS  Google Scholar 

  3. Skulachev, V. P., Anisomov, V. N., Antonenko, Yu. N., Bakeeva, L. E., Chernyak, B. V., Erichev, V. P., Filenko, O. F., Kalinina, N. I., Kapelko, V. I., Kolosova, N. G., Kopnin, B. P., Korshunova, G. A., Lichinitser, M. R., Obukhova, L. A., Pasyukova, E. G., Pisarenko, O. I., Roginsky, V. A., Ruuge, E. K., Senin, I. I., Severina, I. I., Skulachev, M. V., Spivak, I. M., Tashlitsky, B. N., Tkachuk, V. A., Vyssokikh, M. Yu., Jaguzhinsky, L. S., and Zorov, D. B. (2009) Biochim. Biophys. Acta, 1782, 437–461.

    Google Scholar 

  4. Murphy, M. P., and Smith, R. A. J. (2007) Annu. Rev. Pharmacol. Toxicol., 4, 629–656.

    Article  Google Scholar 

  5. Skulachev, V. P. (2007) Biochemistry (Moscow), 72, 1385–1396.

    Article  CAS  Google Scholar 

  6. Green, D. E. (1974) Biochim. Biophys. Acta, 346, 27–78.

    CAS  PubMed  Google Scholar 

  7. Antonenko, Y. N., Avetisyan, A. V., Bakeeva, L. E., Chernyak, B. V., Chertkov, V. A., Domnina, L. V., Ivanova, O. Yu., Izyumov, D. S., Khailova, L. S., Klishin, S. S., Korshunova, G. A., Lyamzaev, K. G., Muntyan, M. S., Nepryakhina, O. K., Pashkovskaya, A. A., Pletjushkina, O. Y., Pustovidko, A. V., Roginsky, V. A., Rokitskaya, T. I., Ruuge, E. K., Saprunova, V. B., Severina, I. I., Simonyan, R. A., Skulachev, I. V., Skulachev, M. V., Sumbatyan, N. V., Sviryaeva, I. V., Tashlitsky, V. N., Vassiliev, J. M., Vyssokikh, M. Y., Yaguzhinsky, L. S., Zamyatnin, A. A., Jr., and Skulachev, V. P. (2008) Biochemistry (Moscow), 73, 1273–1287.

    Article  CAS  Google Scholar 

  8. Neroev, V. V., Archipova, M. M., Bakeeva, L. E., Fursova, A. Zh., Grigoryan, E. N., Grishanova, A. Y., Domdina, E. N., Ivashchenko, Zh. N., Katargina, L. A., KhoroshilovaMaslova, I. P., Kilina, O. V., Kolosova, N. G., Kopenkin, E. P., Korshunov, S. S., Kovaleva, N. A., Novikova, Y. P., Philippov, P. P., Pilipenko, D. I., Robustova, O. V., Saprunova, V. B., Senin, I. I., Skulachev, M. V., Sotnikova, L. F., Stefanova, N. A., Tikhomirova, N. K., Tsapenko, I. V., Shchipanova, A. I., Zinovkin, R. A., and Skulachev, V. P. (2008) Biochemistry (Moscow), 73, 1317–1328.

    Article  CAS  Google Scholar 

  9. Anisimov, V. N., Bakeeva, L. E., Egormin, P. A., Filenko, O. F., Isakova, E. F., Manskikh, V. N., Mikhelson, V. M., Panteleeva, A. A., Pasyukova, E. G., Pilipenko, D. I., Piskunova, T. S., Popovich, I. G., Roshchina, N. V., Rybina, O. Y., Saprunova, V. B., Samoylova, T. A., Semenchenko, A. V., Skulachev, M. V., Spivak, I. M., Tsybul’ko, E. A., Tyndyk, M. L., Vyssokikh, M. Y., Yurova, M. N., Zabezhinsky, M. A., and Skulachev, V. P. (2008) Biochemistry (Moscow), 73, 1329–1342.

    Article  CAS  Google Scholar 

  10. Bakeeva, L. E., Barskov, I. V., Egorov, M. V., Isaev, N. K., Kapelko, V. I., Kazachenko, A. V., Kirpatovsky, V. I., Kozlovsky, S. V., Lakomkin, V. L., Levina, S. B., Pisarenko, O. I., Plotnikov, E. Y., Saprunova, V. B., Serebryakova, L. I., Skulachev, M. V., Stelmashook, E. V., Studneva, I. M., Tskitishvili, O. V., Vasilyeva, A. K., Victorov, I. V., Zorov, D. B., and Skulachev, V. P. (2008) Biochemistry (Moscow), 73, 1288–1299.

    Article  CAS  Google Scholar 

  11. Agapova, L. S., Chernyak, B. V., Domnina, L. V., Dugina, V. B., Efimenko, A. Y., Fetisova, E. K., Ivanova, O. Y., Kalinina, N. I., Khromova, N. V., Kopnin, B. P., Kopnin, P. B., Korotetskaya, M. V., Lichinitser, M. R., Lukashev, A. N., Pletjushkina, O. Y., Popova, E. N., Skulachev, M. V., Shagieva, G. S., Stepanova, E. V., Titova, E. V., Tkachuk, V. A., Vasiliev, J. M., and Skulachev, V. P. (2008) Biochemistry (Moscow), 73, 1300–1316.

    Article  CAS  Google Scholar 

  12. Kovaleva, M. V., Sukhanova,. E. I., Trendeleva,. T. A., Zyl’kova, M. V., Uralskaya,. L. A., Popova, K. M., Saris, N.-E., and Zvyagilskaya, R. A. (2009) J. Bioenerg. Biomembr., 41, 239–249.

    Article  CAS  PubMed  Google Scholar 

  13. Zvyagilskaya, R., Andreishcheva, E., Soares, I. M., Khozin, I., Berhe, A., and Persson, B. L. (2001) J. Basic Microbiol., 41, 283–303.

    Article  Google Scholar 

  14. Zvyagilskaya, R. A., Zelenshchokova, V. F., Ural’skaya, L. A., and Kotel’nikova, A. V. (1981) Biokhimiya, 46, 3–10.

    CAS  Google Scholar 

  15. Bazhenova, E. N., Deryabina, Yu. I., Eriksson, O., Zvyagilskaya, R. A., and Saris, N.-E. L. (1998) J. Biol. Chem., 273, 4372–4377.

    Article  CAS  PubMed  Google Scholar 

  16. Akerman, K. E. O., and Wikstrom, M. K. F. (1976) FEBS Lett., 68, 191–197.

    Article  CAS  PubMed  Google Scholar 

  17. Bradford, M. M. (1976) Anal. Biochem., 72, 248–254.

    Article  CAS  PubMed  Google Scholar 

  18. Chance, B., and Williams, G. R. (1955) Nature, 175, 1120–1121.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071, Moscow, Russia

    E. I. Sukhanova, T. A. Trendeleva & R. A. Zvyagilskaya

Authors
  1. E. I. Sukhanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. A. Trendeleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. A. Zvyagilskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. A. Zvyagilskaya.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 2, pp. 169–176.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sukhanova, E.I., Trendeleva, T.A. & Zvyagilskaya, R.A. Interaction of yeast mitochondria with fatty acids and mitochondria-targeted lipophilic cations. Biochemistry Moscow 75, 139–144 (2010). https://doi.org/10.1134/S0006297910020033

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297910020033

Key words

Search

Navigation