Skip to main content
SpringerLink
Log in

Extracellular yeast-lytic enzyme of the bacterium Lysobacter sp. XL 1

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

An enzyme exhibiting yeast-lytic activity has been isolated from the culture liquid of the bacterium Lysobacter sp. XL. 1. The optimal conditions for the hydrolysis of Saccharomyces cerevisiae cells by the enzyme have been established: 0.15 M sodium acetate buffer, pH 6.0, 50°C. The yeast-lytic activity of the enzyme is inhibited by EDTA, p-chloromercuribenzoate, and phenylmethylsulfonyl fluoride. According to the data of SDS-PAGE, the molecular weight of the protein is 36 kD. The enzyme hydrolyzes casein, hemoglobin, and synthetic peptide Abz-Ala-Ala-Phe-pNA, i.e. it exhibits proteolytic activity. The properties of the enzyme and its molecular weight correspond to those of a previously isolated extracellular metalloproteinase. The N-terminal amino acid sequence of the protein exhibits 67% homology with the N-terminal sequence of achromolysine of Achromobacter lyticus (EC 3.4.24.-).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Abz:

o-aminobenzoate

pNA:

p-nitroaniline

References

  1. Marfenina, O. E. (2002) Priroda, 11, 33–38.

    Google Scholar 

  2. Klis, F. M. (1994) Yeast, 10, 851–869.

    Article  PubMed  CAS  Google Scholar 

  3. Kapteyn, J. C., van den Ende, H., and Klis, F. M. (1999) Biochim. Biophys. Acta, 1426, 373–383.

    PubMed  CAS  Google Scholar 

  4. Kalebina, T. S., and Kulaev, I. S. (2001) Uspekhi Biol. Khim., 41, 105–130.

    CAS  Google Scholar 

  5. Iranzo, M., Aguado, C., Palotti, C., Canizares, J. V., and Vormeneo, S. (2000) Res. Microbiol., 4, 227–232.

    Google Scholar 

  6. Obata, T., Iwata, H., and Namba, Y. (1977) Agric. Biol. Chem., 41, 2387–2394.

    CAS  Google Scholar 

  7. Phaff, H. J. (1977) in Advances in Chemistry (Feeney, R. E., and Whitaker, J. R., eds.) Vol. 160, American Chemical Society, Washington, pp. 244–282.

    Google Scholar 

  8. Kalebina, T. S., Rudenskaya, G. N., Selyakh, I. O., Khodova, O. M., Chestukhina, G. G., Stepanov, V. M., and Kulaev, I. S. (1988) Appl. Microbiol. Biotechnol., 28, 531–536.

    Article  CAS  Google Scholar 

  9. Sivan, A., and Chet, I. (1989) J. Gen. Microbiol., 135, 675–682.

    CAS  Google Scholar 

  10. Van Tilburg, A.-U. B., and Thomas, M. D. (1993) Appl. Environ. Microbiol., 59, 236–242.

    PubMed  Google Scholar 

  11. Velazhahan, R., Samiyappan, R., and Vidhysekaran, P. (2000) Phytoparasitica, 28, 131–139.

    Article  CAS  Google Scholar 

  12. Melent’ev, A. I., Aktuganov, G. E., and Galimzyanova, N. F. (2001) Mikrobiologiya, 70, 548–552.

    Google Scholar 

  13. Taira, T., Ohnuma, T., Yamagami, T., and Aso, Y. (2002) Biosci. Biotechnol. Biochem., 66, 970–977.

    Article  PubMed  CAS  Google Scholar 

  14. Bacteriolytic Complex, Method of Its Isolation, and Strain for Realization of the Method, RF Patent No. 2193063; Byul. Izobret. (2002) No. 32.

  15. Stepnaya, O. A., Ledova, L. A., and Kulaev, I. S. (1999) Uspekhi Biol. Khim., 39, 327–354.

    CAS  Google Scholar 

  16. Stepnaya, O. A., Begunova, E. A., Tsfasman, I. M., and Kulaev, I. S. (1996) Biochemistry (Moscow), 61, 471–476.

    Google Scholar 

  17. Stepnaya, O. A., Begunova, E. A., and Kulaev, I. S. (1996) Biochemistry (Moscow), 61, 477–482.

    Google Scholar 

  18. Begunova, E. A., Lysanskaya, V. Ya., Stepnaya, O. A., and Kulaev, I. S. (2003) Biochemistry (Moscow), 68, 735–739.

    Article  CAS  Google Scholar 

  19. Stepnaya, O. A., Tsfasman, I. M., Logvina, I. A., Ryazanova, L. P., Muranova, T. A., and Kulaev, I. S. (2005) Biochemistry (Moscow), 70, 1031–1037.

    Article  CAS  Google Scholar 

  20. Ryazanova, L. P., Stepnaya, O. A., Suzina, N. E., and Kulaev, I. S. (2005) Proc. Biochem., 40, 557–564.

    Article  CAS  Google Scholar 

  21. Krupyanko, V. I., Kudryavtseva, A. I., Valiakhmetov, A. Ya., Severin, A. I., Abramochkin, G. V., Zyakun, A. M., Lysogorskaya, E. N., Filippova, I. Yu., Kulaev, I. S., and Stepanov, V. M. (1989) Biokhimiya, 54, 1140–1149.

    CAS  Google Scholar 

  22. Nelson, N. J. (1944) J. Biol. Chem., 153, 375–380.

    CAS  Google Scholar 

  23. Laemmli, U. K. (1970) Nature, 227, 680–685.

    Article  PubMed  CAS  Google Scholar 

  24. Bradford, M. M. (1976) Analyt. Biochem., 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  25. Severin, A. I., Stepnaya, O. A., and Kulaev, I. S. (1986) Biokhimiya, 51, 748–753.

    Google Scholar 

  26. Kitamura, K. (1982) Agric. Biol. Chem., 46, 2093–2099.

    CAS  Google Scholar 

  27. Stepanov, V. M., Rudenskaya, G. N., Nesterova, N. G., Kupriyanova, T. I., Khokhlova, Yu. M., Usaite, I. A., Loginova, L. G., and Timokhina, E. A. (1980) Biokhimiya, 45, 1871–1880.

    CAS  Google Scholar 

  28. Funatsu, M., Oh, H., Aizono, J., and Shimoda, T. (1978) Agric. Biol. Chem., 42, 1975–1977.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290, Pushchino, Moscow Region, Russia

    O. A. Stepnaya, I. M. Tsfasman, I. A. Chaika & I. S. Kulaev

  2. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 7, 142290, Pushchino, Moscow Region, Russia

    T. A. Muranova

Authors
  1. O. A. Stepnaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. M. Tsfasman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. A. Chaika
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. A. Muranova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. S. Kulaev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. A. Stepnaya.

Additional information

Original Russian Text © O.A. Stepnaya, I.M. Tsfasman, I.A. Chaika, T.A. Muranova, I.S. Kulaev, 2008, published in Biokhimiya, 2008, Vol. 73, No. 3, pp. 381–387.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stepnaya, O.A., Tsfasman, I.M., Chaika, I.A. et al. Extracellular yeast-lytic enzyme of the bacterium Lysobacter sp. XL 1. Biochemistry Moscow 73, 310–314 (2008). https://doi.org/10.1134/S0006297908030115

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297908030115

Key words

Search

Navigation