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Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase

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Abstract

Indole-3-glycerol phosphate synthase (IGPS) plays an important role in the survival of Mycobacterium tuberculosis. The trpC gene, encoding IGPS, is essential for the growth of M. tuberculosis. It was expressed at the transcriptional level in cultured M. tuberculosis. The recombinant IGPS with an added His-tag was purified. The His-tag was found to have a small effect on the biochemical properties of IGPS. IGPS is a monofunctional enzyme in M. tuberculosis. Recombinant IGPS has considerable β-pleated sheet and is relatively compact. The enzyme activity is significantly inhibited by denaturants and antibiotics, suggesting that IGPS may be a novel potential drug target of M. tuberculosis.

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Abbreviations

IGP:

indole-3-glycerol phosphate

IGPS:

indole-3-glycerol phosphate synthase

CdRP:

1-(o-carboxyphenylamino)-1-deoxyribulose-5′-phosphate

PRA:

N-(5′-phosphoribosyl)anthranilate

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, PR China

    Yanping Yang, Min Zhang, Hongmei Zhang, Jianqiang Lei, Ruiliang Jin, Shengfeng Xu, Jialing Bao, Lu Zhang & Honghai Wang

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  1. Yanping Yang
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  2. Min Zhang
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  3. Hongmei Zhang
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  4. Jianqiang Lei
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  5. Ruiliang Jin
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  6. Shengfeng Xu
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  7. Jialing Bao
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  8. Lu Zhang
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  9. Honghai Wang
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Correspondence to Honghai Wang.

Additional information

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-347, May 1, 2005.

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Yang, Y., Zhang, M., Zhang, H. et al. Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase. Biochemistry (Moscow) 71 (Suppl 1), S38–S43 (2006). https://doi.org/10.1134/S0006297906130062

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  • DOI: https://doi.org/10.1134/S0006297906130062

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