Abstract
Carbonic anhydrase activities of pea thylakoids as well as thylakoid fragments enriched either in Photosystem 1 (PS1-membranes) or Photosystem 2 (PS2-membranes) were studied. The activity of PS1-membranes if calculated on chlorophyll basis was much higher than the activity of PS2-membranes. Acetazolamide, a non-permeable inhibitor of carbonic anhydrases, increased carbonic anhydrase activity of PS2-membranes at concentrations lower than 10−6 M and suppressed this activity only at higher concentrations. A lipophilic inhibitor of carbonic anhydrases, ethoxyzolamide, effectively suppressed the carbonic anhydrase activity of PS2-membranes (I 50 = 10−9 M). Carbonic anhydrase activity of PS1-membranes was suppressed alike by both inhibitors (I 50 = 10−6 M). In the course of the electrophoresis of PS2-membranes treated with n-dodecyl-β-maltoside “high-molecular-mass” carbonic anhydrase activity was revealed in the region corresponding to core-complex of this photosystem. Besides, carbonic anhydrase activity in the region of low-molecular-mass proteins was discovered in the course of such an electrophoresis of both PS2-and PS1-membranes. These low-molecular-mass carbonic anhydrases eluted from corresponding gels differed in sensitivity to specific carbonic anhydrase inhibitors just the same as PS1-membranes versus PS2-membranes. The results are considered as evidence for the presence in the thylakoid membranes of three carriers of carbonic anhydrase activity.
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Abbreviations
- AA:
-
acetazolamide
- BTB:
-
bromthymol blue
- CA:
-
carbonic anhydrase
- Chl:
-
chlorophyll
- DM:
-
n-dodecyl-β-maltoside
- EZ:
-
ethoxyzolamide
- PS1, PS2:
-
Photosystems 1 and 2
- PS1-and PS2-membranes:
-
fragments of thylakoid membranes enriched with pigment—protein complexes of corresponding photosystems
- PMSF:
-
phenylmethylsulfonyl fluoride
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Published in Russian in Biokhimiya, 2006, Vol. 71, No. 5, pp. 651–659.
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Ignatova, L.K., Rudenko, N.N., Khristin, M.S. et al. Heterogeneous origin of carbonic anhydrase activity of thylakoid membranes. Biochemistry (Moscow) 71, 525–532 (2006). https://doi.org/10.1134/S0006297906050099
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DOI: https://doi.org/10.1134/S0006297906050099