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Assessment of the Biotechnological Potential of Cyanobacterial and Microalgal Strains from IPPAS Culture Collection

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Abstract

A search for strains capable of the simultaneous production of high amounts of several biologically valuable compounds and/or high biomass productivity has been carried out. The growth characteristics and biochemical composition of 12 microalgal and cyanobacterial strains from the IPPAS Collection were studied at the exponential and stationary growth phases. All of the strains had high growth rates (a doubling time of 6–22 h). The strains Cyanobacterium sp. IPPAS B-1200, Chlorella sp. IPPAS C-1210, Nannochloris sp. IPPAS C-1509, Cyanidium caldarium IPPAS P-510, and Vischeria sp. IPPAS H-242 demonstrated the highest biotechnological potential and can be used for the production of various types of biofuel, pigments, and feed and food additives, including those with a high content of eicosapentaenoic acid (20 : 5 Δ5, 8,11, 14, 17).

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Funding

This work was supported by a grant from the Russian Science Foundation (no. 14-14-00904).

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    M. A. Sinetova, R. A. Sidorov, A. Yu. Starikov, A. S. Voronkov, A. S. Medvedeva, Z. V. Krivova, D. V. Bachin, V. S. Bedbenov, D. A. Gabrielyan & D. A. Los

  2. Lomonosov Northern (Arctic) Federal University, 163002, Arkhangelsk, Russia

    M. S. Pakholkova

  3. Al-Farabi Kazakh National University, 050040, Almaty, Republic of Kazakhstan

    B. K. Zayadan & K. Bolatkhan

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  1. M. A. Sinetova
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  2. R. A. Sidorov
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  4. A. S. Voronkov
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Correspondence to M. A. Sinetova.

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Translated by I. Gordon

Abbreviations: ARA—arachidonic acid; Car—content of total carotenoids; dw—dry weight; Chl a(b)—chlorophyll a(b) content; EPA—eicosapentaenoic acid; EPS—exopolysaccharide(s); FA—fatty acid; FAME—fatty acid methyl ester; FFA—free fatty acid; OD750—optical density at a wavelength of 750 nm; Pf—final productivity; PUFA—polyunsaturated fatty acid; SDS—sodium dodecyl sulfate; Td—biomass doubling time; TAG—triacylglycerol; TLs—total lipids; UI—unsaturation index; μ—specific growth rate; μmax—maximal specific growth rate at the exponential growth phase.

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Sinetova, M.A., Sidorov, R.A., Starikov, A.Y. et al. Assessment of the Biotechnological Potential of Cyanobacterial and Microalgal Strains from IPPAS Culture Collection. Appl Biochem Microbiol 56, 794–808 (2020). https://doi.org/10.1134/S0003683820070030

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