Abstract
The genes frdAB and sdhAB, which encode components of fumarate reductase and succinate dehydrogenase, have been deleted in a recombinant E. coli strain with the inactivated pathways of mixed-acid fermentation and a modified system of glucose transport and phosphorylation upon the heterological expression of the pyruvate carboxylase gene. Under anaerobic conditions, the parental strain efficiently converted glucose to succinic acid without synthesizing notable amounts of fumaric or malic acid. Upon individual deletion of the frdAB genes, the mutant strain fermented glucose to succinic acid less efficiently secreting notable amounts of malic and fumaric acids. Individual deletion of the sdhAB genes in the parental strain did not significantly affect the formation of the main fermentation end-product. The combined inactivation of fumarate reductase and succinate dehydrogenase in the constructed strain enhanced the anaerobic conversion of glucose to fumaric and malic acids with the activation of the glyoxylate bypass and decrease in the contribution of the reductive branch of the TCA cycle to the formation of the target products.
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Abbreviations
- HPLC:
-
high performance liquid chromatography
- CL:
-
culture liquid
- PCR:
-
polymerase chain reaction
- TCA cycle:
-
tricarboxylic acid cycle
- AceA:
-
isocitrate lyase
- AceB:
-
malate synthase
- AcnA, AcnB:
-
aconitases
- FumA, FumB, FumC:
-
isoenzymes of fumarase
- FrdABCD:
-
fumarate reductase enzymatic complex
- GltA:
-
citrate synthase
- Mdh:
-
malate dehydrogenase
- NADH:
-
reduced nicotinamide adenine dinucleotide
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Original Russian Text © A.Yu. Skorokhodova, A.Yu. Gulevich, V.G. Debabov, 2015, published in Biotekhnologiya, 2015, No. 6, pp. 9–15.
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Skorokhodova, A.Y., Gulevich, A.Y. & Debabov, V.G. Anaerobic biosynthesis of intermediates of reductive branch of tricarboxylic acids cycle by Escherichia coli strains with inactivated frdAB and sdhAB genes. Appl Biochem Microbiol 52, 679–684 (2016). https://doi.org/10.1134/S0003683816070061
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DOI: https://doi.org/10.1134/S0003683816070061