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Synthesis of recombinant gamma interferons resistant to proteolysis in the yeast Pichia pastoris

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Abstract

Genes encoding truncated versions of bovine and chicken gamma interferon genes lacking predicted protease cleavage sites at the C-terminus were constructed and expressed in the yeast Pichia pastoris. The recombinant proteins possessed increased stability in comparison with the corresponding wild-type gamma interferons while retaining biological activity. The recombinant strains provide a useful tool for the purification of bovine and chicken gamma interferons for their use in veterinary applications.

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, 199034, Russia

    M. A. Tsygankov, A. E. Zobnina & M. V. Padkina

Authors
  1. M. A. Tsygankov
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  2. A. E. Zobnina
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  3. M. V. Padkina
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Correspondence to M. A. Tsygankov.

Additional information

Original Russian Text © M.A. Tsygankov, A.E. Zobnina, M.V. Padkina, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 4, pp. 429–436.

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Tsygankov, M.A., Zobnina, A.E. & Padkina, M.V. Synthesis of recombinant gamma interferons resistant to proteolysis in the yeast Pichia pastoris . Appl Biochem Microbiol 50, 387–393 (2014). https://doi.org/10.1134/S0003683814040127

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  • DOI: https://doi.org/10.1134/S0003683814040127

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