Abstract
The peculiarities of the acidic hydrolysis of chitin-glucan complexes (ChGCs) of higher fungi were studied, and the technology for the isolation and purification of D-(+)-glucosamine hydrochloride of high purity from hydrolysate was developed. The composition, properties, and purity of the product were analyzed by a combination of physicochemical methods. The yield of the final product was 20–60%, depending on the chitin content in ChGC samples. The obtained amino sugar was a white crystalline odorless powder readily soluble in water, slightly soluble in 95% ethanol, and insoluble in chloroform and other organic solvents, and on the basic parameters corresponded to the standard D-(+)-glucosamine hydrochloride.
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References
Kochetkov, N.K., Bochkov, A.F., and Dmitriev, B.A., Khimiya uglevodov (Carbohydrate Chemistry), Moscow: Khimiya, 1967.
Lazur’evskii, G.V., Terent’eva, I.V., and Shamshurin, A.A., Prakticheskie raboty po khimii prirodnykh soedinenii (Chemistry of Natural Compounds: Practical Works), Moscow: Vysshaya shkola, 1961.
Verbruggen, G., Goemaere, S., and Veys, E.M., Clin. Rheum., 2002, vol. 21, pp. 231–243.
Kornilov, E.N., Afinogenov, G.E., Red’ko, K.G., Kompantsev, V.A., Vergeichik, E.N., Anisimova, L.O., Netyl’ko, G.I., and Afinogenova, A.G., Travmatol. Ortoped. Rossii, 2003, no. 3, pp. 21–23.
Gatterman, L. and Viland, G., Prakticheskie raboty po organicheskoi khimii (Organic Chemistry: Practical Works), Rodionov, V.M., Ed., Moscow: Goskhimizdat, 1948.
Feofilova, E.P., Tereshina, V.M., and Memorskaya, A.S., Mikrobiologiya, 1995, vol. 64, no. 1, pp. 27–31.
Markov, S.F., Farmatsiya (B@lg.), 1964, vol. 14, no. 2, pp. 30–32.
Ivshin, V.P., Artamonova, S.D., Ivshina, T.N., and Sharnina, F.F., Vysokomol. Soedin., Ser. B, 2007, vol. 49, no. 12, pp. 2215–2222.
Ivshina, T.N., Artamonova, S.D., Ivshin, V.P., and Sharnina, F.F., Appl. Biochem. Microbiol., 2009, vol. 45, no. 3, pp. 348–353.
Metody khimii uglevodov (Methods of Carbohydrate Chemistry), Kochetkov, N.K., Ed., Moscow: Mir, 1967.
Elson, L.A. and Morgan, W.T., J. Biochem., 1933, vol. 27, pp. 1824–1828.
Kazitsyna, L.A. and Kupletskaya, N.B., Primenenie UF-, IK- i YaMR-spektroskopii v organicheskoi khimii (Application of UV, IR, and NMR Spectroscopy in Organic Chemistry), Moscow: Vysshaya shkola, 1971.
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Original Russian Text © S.D. Artamonova, F.F. Sharnina, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 4, pp. 417–422.
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Artamonova, S.D., Sharnina, F.F. Isolation of D-glucosamine from chitin-glucan complexes. Appl Biochem Microbiol 49, 426–431 (2013). https://doi.org/10.1134/S0003683813030046
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DOI: https://doi.org/10.1134/S0003683813030046