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Catalytic properties of Rhodotorula aurantiaca KM-1 phenylalanine ammonia-lyase

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Abstract

L-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) of the Rhodotorula aurantiaca strain KM-1 deaminates L-phenylalanine according to the Michaelis-Menten kinetics with K M 1.75 ± 0.44 mM and V max 3.01 ± 0.43 units/mg. The enzyme is competitively inhibited by D-phenylalanine with K i 3.38 ± 0.32 mM. The Michaelis-Menten kinetics was analyzed, the inhibition type (competitive, noncompetitive, and mixed) was identified, and corresponding kinetic parameters were calculated using the computer programs written in Gauss 4.0. PAL was most stable at pH 6.55 and lacked approximately 50% of its activity after incubation at 57°C for 15 min. The yield of L-phenylalanine increased in the presence of mercaptoethanol, sodium ethylenediaminetetraacetate (EDTA), and ascorbic acid. The effects of EDTA and ascorbic acid were additive.

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Authors and Affiliations

  1. Yerevan State University, Yerevan, 0025, Armenia

    I. L. Bazukyan, A. E. Vardanyan & Yu. G. Popov

  2. CAO SRI Biotechnology, Yerevan, 0056, Armenia

    A. A. Hambardzumyan

  3. Georisk Research Company, National Academy of Sciences of the Republic of Armenia, Yerevan, 0019, Armenia

    P. V. Tozalakyan

Authors
  1. I. L. Bazukyan
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  2. A. E. Vardanyan
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  3. A. A. Hambardzumyan
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  4. P. V. Tozalakyan
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  5. Yu. G. Popov
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Correspondence to I. L. Bazukyan.

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Original Russian Text © I.L. Bazukyan, A.E. Vardanyan, A.A. Hambardzumyan, P.V. Tozalakyan, Yu.G. Popov, 2009, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 1, pp. 23–27.

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Bazukyan, I.L., Vardanyan, A.E., Hambardzumyan, A.A. et al. Catalytic properties of Rhodotorula aurantiaca KM-1 phenylalanine ammonia-lyase. Appl Biochem Microbiol 45, 17–21 (2009). https://doi.org/10.1134/S0003683809010037

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  • DOI: https://doi.org/10.1134/S0003683809010037

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