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Natural and recombinant luminescent microorganisms in biotoxicity testing of mineral waters

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Abstract

We have developed methods of biotesting mineral waters involving use of natural or recombinant luminescent strains with elimination of the effect of degree of mineralization and pH. To overcome the adverse effect of high salt concentrations, disguising the action of chemical pollutants, a special method of mineral water sample preparation is proposed. In this method, the marine luminescent bacterium Photobacterium phosphoreum (Microbiosensor B-17 677f) is used as a test object. Samples to be analyzed are supplemented with NaCl depending on their natural degree of mineralization to adjust it to 30 g/l. Another approach, more universal and efficient, involves pH adjustment in the samples to 7.5. This value is suitable for application of both Microbiosensor B-17 677f and the recombinant Escherichia coli strain harboring the cloned lux operon of P. leiognathi (Ecolum-9). It has been shown that this treatment, retaining the natural luminescence level of the bacterial biosensors, allows bioluminescent detection of exogenous pollutants added to the samples, including benzene and Cr(VI).

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Authors and Affiliations

  1. Orenburg State University, Orenburg, 460018, Russia

    D. G. Deryabin & E. S. Aleshina

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  1. D. G. Deryabin
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  2. E. S. Aleshina
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Correspondence to D. G. Deryabin.

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Original Russian Text © D.G. Deryabin, E.S. Aleshina, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 4, pp. 417–421.

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Deryabin, D.G., Aleshina, E.S. Natural and recombinant luminescent microorganisms in biotoxicity testing of mineral waters. Appl Biochem Microbiol 44, 378–381 (2008). https://doi.org/10.1134/S0003683808040078

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  • DOI: https://doi.org/10.1134/S0003683808040078

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