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Immobilization of Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon supports

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Abstract

Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, was immobilized by adsorption on carbon supports differing in structure and porosity. The adsorption capacity of the supports towards cells, the substrate of the nitrile hydratase reaction (acrylonitrile), and the product (acrylamide) was studied. Also, the effect of immobilization on nitrile hydratase activity of bacteria was investigated, and the operational stability of the immobilized biocatalyst was determined. It was shown that crushed and granulated active coals were more appropriate for immobilization than fibrous carbon adsorbents.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Uralian Division of the Russian Academy of Sciences, Perm, 614081, Russia

    A. Yu. Maksimov, Yu. G. Maksimova, M. V. Kuznetsova & V. A. Demakov

  2. OAO Sorbent, Perm, 614113, Russia

    V. F. Olontsev

Authors
  1. A. Yu. Maksimov
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  2. Yu. G. Maksimova
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  3. M. V. Kuznetsova
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  4. V. F. Olontsev
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  5. V. A. Demakov
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Original Russian Text © A.Yu. Maksimov, Yu.G. Maksimova, M.V. Kuznetsova, V.F. Olontsev, V.A. Demakov, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 2, pp. 193–198.

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Maksimov, A.Y., Maksimova, Y.G., Kuznetsova, M.V. et al. Immobilization of Rhodococcus ruber strain gt1, possessing nitrile hydratase activity, on carbon supports. Appl Biochem Microbiol 43, 173–177 (2007). https://doi.org/10.1134/S000368380702007X

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  • DOI: https://doi.org/10.1134/S000368380702007X

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