Abstract
Our study confirms the presence of chitinolytic, chitosanolytic, and deacetylase activities in the hepatopancreas of the red king crab, related to the specific diet of this species. The maximum rate of chitin/chitosan hydrolysis by an enzyme preparation from crab hepatopancreas occurs at 36.5–37.0°C. Two pH optimums have been found for the enzymatic reaction under mildly alkaline and acidic conditions for both exo-and endochitinase activities. The enzyme preparation is most affine to partly deacetylated chitin with an acetylation degree within 40–50%.
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Original Russian Text © V.Yu. Novikov, V.A. Mukhin, K.S. Rysakova, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 2, pp. 178–183.
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Novikov, V.Y., Mukhin, V.A. & Rysakova, K.S. Properties of chitinolytic enzymes from the hepatopancreas of the red king crab (Paralithodes camtschaticus). Appl Biochem Microbiol 43, 159–163 (2007). https://doi.org/10.1134/S0003683807020056
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DOI: https://doi.org/10.1134/S0003683807020056