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An electrochemical method for measuring metabolic activity and counting cells

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Abstract

An express electrochemical method for determining the metabolic activity of live cells based on the possibility of an electron exchange between an electrode and elements of the biological electron transfer chain in the presence of a mediator is proposed. This method is useful for studying any live cells (animal, plant, and microbial), including anaerobic, dormant, and spore cells. The sample preparation and measurement itself does not take more than 30 min. The detection limit in a volume of 15 ml amounts to 105 cells/ml. The applicability of the assessment method of the metabolic activity level during the transition of the bacteria Mycobacterium smegmatis into an uncultivable dormant state was demonstrated. This method is of special value for medicine and environmental control, detecting latent forms of pathogens. An optimal combination of the methods for the express analysis of latent pathogens is proposed.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    B. A. Kuznetsov, M. T. Khlupova, S. V. Shleev, A. S. Kaprel’yants & A. I. Yaropolov

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  1. B. A. Kuznetsov
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  2. M. T. Khlupova
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  3. S. V. Shleev
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  4. A. S. Kaprel’yants
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  5. A. I. Yaropolov
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Additional information

Original Russian Text © B.A. Kuznetsov, M.T. Khlapova, S.V. Shleev, A.S. Kaprel’yants, A.I. Yaropolov, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 5, pp. 599–608.

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Kuznetsov, B.A., Khlupova, M.T., Shleev, S.V. et al. An electrochemical method for measuring metabolic activity and counting cells. Appl Biochem Microbiol 42, 525–533 (2006). https://doi.org/10.1134/S0003683806050140

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  • DOI: https://doi.org/10.1134/S0003683806050140

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