Abstract
Polycondensation of a catalase (EC 1.11.1.6) with glutaraldehyde in order to stabilize the quaternary structure of an enzyme, maintain its activity, and protect it from thermal denaturation was studied. Synthesis showed a superequivalent utilization of the aldehyde groups relative to the catalase amine groups, as a result of the formation of glutaraldehyde oligomers linked to the enzyme.
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Original Russian Text © R.N. Mishaeva, L.R. Gudkin, N.P. Kuznetsova, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 4, pp. 404–408.
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Mishaeva, R.N., Gudkin, L.R. & Kuznetsova, N.P. Modification of a catalase by glutaraldehyde. Appl Biochem Microbiol 42, 354–359 (2006). https://doi.org/10.1134/S0003683806040028
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DOI: https://doi.org/10.1134/S0003683806040028