Modeling photo-bleaching kinetics to map local variations in rod rhodopsin density

M. Ehler; J. Dobrosotskaya; E. J. King; W. Czaja; R. F. Bonner

doi:10.1117/12.878421

9 March 2011 Modeling photo-bleaching kinetics to map local variations in rod rhodopsin density

M. Ehler, J. Dobrosotskaya, E. J. King, W. Czaja, R. F. Bonner

Proceedings Volume 7963, Medical Imaging 2011: Computer-Aided Diagnosis; 79633R (2011) https://doi.org/10.1117/12.878421
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

Localized rod photoreceptor and rhodopsin losses have been observed in post mortem histology both in normal aging and in age-related maculopathy. We propose to noninvasively map local rod rhodopsin density through analysis of the brightening of the underlying lipofuscin autofluorescence (LAF) in confocal scanning laser ophthalmoscopy (cSLO) imaging sequences starting in the dark adapted eye. The detected LAF increases as rhodopsin is bleached (time constant ≈ 25sec) by the average retinal irradiance of the cSLO 488nm laser beam. We fit parameters of analytical expressions for the kinetics of rhodopsin bleaching that Lamb validated using electroretinogram recordings in human. By performing localized (≈ 100μm) kinetic analysis, we create high resolution maps of the rhodopsin density. This new noninvasive imaging and analysis approach appears well-suited for measuring localized changes in the rod photoreceptors and correlating them at high spatial resolution with localized pathological changes of the retinal pigment epithelium (RPE) seen in steady-state LAF images.

Citation Download Citation

M. Ehler, J. Dobrosotskaya, E. J. King, W. Czaja, and R. F. Bonner "Modeling photo-bleaching kinetics to map local variations in rod rhodopsin density", Proc. SPIE 7963, Medical Imaging 2011: Computer-Aided Diagnosis, 79633R (9 March 2011); https://doi.org/10.1117/12.878421

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