Hyperspectral fluorescence tomography of quantum dots

Alexander D. Klose

doi:10.1117/12.875021

17 February 2011 Hyperspectral fluorescence tomography of quantum dots

Alexander D. Klose

Proceedings Volume 7896, Optical Tomography and Spectroscopy of Tissue IX; 78962P (2011) https://doi.org/10.1117/12.875021
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Hyperspectral excitation-resolved fluorescence tomography (HEFT) exploits the spectrally-dependent absorption properties of biological tissue for recovering the unknown three-dimensional (3D) fluorescent reporter distribution inside tissue. Only a single light source with macro-illumination and wavelength-discrimination is required for the purpose of light emission stimulation and 3D image reconstruction. HEFT is built on fluorescent sources with a relatively broad spectral absorption profile (quantum dots) and a light propagation model for strongly absorbing tissue between wavelengths 560 nm and 660 nm (simplified spherical harmonics - SPN, - equations). The measured partial current of fluorescence light is cast into an algebraic system of equations, which is solved for the unknown quantum dot distribution with an expectation-maximization (EM) method. HEFT requires no source-detector multiplexing for 3D image reconstruction and, hence, offers a technologically simple design.

Citation Download Citation

Alexander D. Klose "Hyperspectral fluorescence tomography of quantum dots", Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78962P (17 February 2011); https://doi.org/10.1117/12.875021

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