Using multimodal femtosecond CARS imaging to determine plaque burden in luminal atherosclerosis

Alex C-T. Ko; Leila B. Mostaço-Guidolin; Andrew Ridsdale; Adrian F. Pegoraro; Michael S. D. Smith; Aaron Slepkov; Mark D. Hewko; Elicia K. Kohlenberg; Bernie Schattka; Albert Stolow; Michael G. Sowa

doi:10.1117/12.873867

22 February 2011 Using multimodal femtosecond CARS imaging to determine plaque burden in luminal atherosclerosis

Alex C-T. Ko, Leila B. Mostaço-Guidolin, Andrew Ridsdale, Adrian F. Pegoraro, Michael S. D. Smith, Aaron Slepkov, Mark D. Hewko, Elicia K. Kohlenberg, Bernie Schattka, Albert Stolow, Michael G. Sowa

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790318 (2011) https://doi.org/10.1117/12.873867
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Luminal atherosclerosis imaging was demonstrated by multimodal femtosecond CARS microscopy (MM-CARS). Using a myocardial infarction-prone rabbit model of atherosclerosis, this study demonstrated the utility of multimodal CARS imaging in determining atherosclerotic plaque burden through two types of image analysis procedures. Firstly, multimodal CARS images were evaluated using a signal-intensity parameter based on intensity changes derived from the multi-channel data (e.g. TPEF, SHG and CARS) to classify plaque burden within the vessel. Secondly, the SHG images that mainly correspond to collagen fibrils were evaluated using a texture analysis model based on the first-order statistical (FOS) parameters of the image histogram. Correlation between FOS parameters of collagen images with atherosclerosis plaque burden was established. A preliminary study of using spectroscopic CARS in identifying the different lipid components within the plaque was also discussed.

Citation Download Citation

Alex C-T. Ko, Leila B. Mostaço-Guidolin, Andrew Ridsdale, Adrian F. Pegoraro, Michael S. D. Smith, Aaron Slepkov, Mark D. Hewko, Elicia K. Kohlenberg, Bernie Schattka, Albert Stolow, and Michael G. Sowa "Using multimodal femtosecond CARS imaging to determine plaque burden in luminal atherosclerosis", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790318 (22 February 2011); https://doi.org/10.1117/12.873867

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available