Force measurements with a translating holographic optical trap

Louis T McLane; Keith M. Carroll; Jan Scrimgeour; Mauricio D. Bedoya; Anthony Kramer; Jennifer E. Curtis

doi:10.1117/12.863549

27 August 2010 Force measurements with a translating holographic optical trap

Louis T McLane, Keith M. Carroll, Jan Scrimgeour, Mauricio D. Bedoya, Anthony Kramer, Jennifer E. Curtis

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77621J (2010) https://doi.org/10.1117/12.863549
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Force measurements made with a translating holographic optical trap (HOT) of a viscous and a viscoelastic medium are investigated. In purely viscous media, Stokes drag cannot be measured with a translating HOT with established methods. In the viscoelastic system of the pericellular coat, the standard force curves generated by a fixed optical trap coupled with a moving stage can reliably be reproduced by translating HOT experiments. The viscoelastic cell coat provides an example where slow relaxation dynamics makes force measurements relatively insensitive to differences between measurements. These preliminary studies suggest that when the relaxation time scale of a system is much slower than the time scale of the HOT updates, translating HOTs can be reliably used to make force measurements on a viscoelastic, non-equilibrium system.

Citation Download Citation

Louis T McLane, Keith M. Carroll, Jan Scrimgeour, Mauricio D. Bedoya, Anthony Kramer, and Jennifer E. Curtis "Force measurements with a translating holographic optical trap", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621J (27 August 2010); https://doi.org/10.1117/12.863549

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