Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system

Jong-ryul Choi; Jong Hwan Sung; Michael L. Shuler; Donghyun Kim

doi:10.1117/12.841729

24 February 2010 Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system

Jong-ryul Choi, Jong Hwan Sung, Michael L. Shuler, Donghyun Kim

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75680X (2010) https://doi.org/10.1117/12.841729
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We have investigated a confocal fluorescence detection system for 3D cultured mammalian cells in a microfluidic cell culture analog system. For direct measurement of metabolic changes in the microfluidic cell culture systems using 3D cell cultures, we constructed a compact and portable confocal fluorescence detection system based on discrete optical components. The confocal detection system was designed to provide a depth scan in situ without lateral imaging for fast scanning and acquisition of average fluorescence variation associated with a large number of cells in 3D cultures of microfluidic devices. The results were consistent with the data obtained by a standard confocal microscope and suggest the potential for monitoring cell dynamics in 3D cell-based assays.

Citation Download Citation

Jong-ryul Choi, Jong Hwan Sung, Michael L. Shuler, and Donghyun Kim "Confocal fluorescence detection for 3D cultured mammalian cells in a microfluidic cell culture system", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680X (24 February 2010); https://doi.org/10.1117/12.841729

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