Versatile photonic crystal fiber-enabled source for multi-modality biophotonic imaging beyond conventional multiphoton microscopy

Haohua Tu; Stephen A. Boppart

doi:10.1117/12.841298

26 February 2010 Versatile photonic crystal fiber-enabled source for multi-modality biophotonic imaging beyond conventional multiphoton microscopy

Haohua Tu, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692D (2010) https://doi.org/10.1117/12.841298
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In a typical multiphoton microscope, the majority of the laser power from the near-IR pulsed source is thrown away in order to prevent photo-damage on biological samples. It is thus desirable to convert this wasted power into other wavelengths through nonlinear fiber optics to accommodate imaging modalities beyond conventional multiphoton microscopy. Here we present a prototypical source that accommodates the requirements for laser scanning confocal microscopy, fluorescence lifetime imaging microscopy, stimulated emission microscopy, coherent anti-Stokes Raman microscopy/spectroscopy, transient absorption microscopy, and optical coherence tomography/microscopy. Such versatile source consists of dispersion-engineered photonic crystal fibers simultaneously pumped by a compact ~1040 nm ultrafast ytterbium laser.

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Haohua Tu and Stephen A. Boppart "Versatile photonic crystal fiber-enabled source for multi-modality biophotonic imaging beyond conventional multiphoton microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692D (26 February 2010); https://doi.org/10.1117/12.841298

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