FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope

V. Devauges; P. Blandin; J. C. Cossec; S. Lécart; C. Marquer; M. C. Potier; F. Druon; P. Georges; S. Lévêque-Fort

doi:10.1117/12.831537

1 July 2009 FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope

V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M. C. Potier, F. Druon, P. Georges, S. Lévêque-Fort

Author Affiliations +

Proceedings Volume 7367, Advanced Microscopy Techniques; 736711 (2009) https://doi.org/10.1117/12.831537
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We present the development of a time resolved TIRF microscope illuminated by a supercontinuum laser source. It permits to perform wide-field fluorescence lifetime imaging of neurobiological processes at the plasma membrane with subwavelength axial resolution.

Citation Download Citation

V. Devauges, P. Blandin, J. C. Cossec, S. Lécart, C. Marquer, M. C. Potier, F. Druon, P. Georges, and S. Lévêque-Fort "FRET detection for neurobiological applications using a total internal reflection fluorescence lifetime imaging microscope", Proc. SPIE 7367, Advanced Microscopy Techniques, 736711 (1 July 2009); https://doi.org/10.1117/12.831537

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