Behaviour of enzyme molecules under piezo-electric vibrations

Amritsar Jeetender; Ion Stiharu; Muthukumaran Packirisamy; Vahé Nerguizian

doi:10.1117/12.708257

8 September 2006 Behaviour of enzyme molecules under piezo-electric vibrations

Amritsar Jeetender, Ion Stiharu, Muthukumaran Packirisamy, Vahé Nerguizian

Proceedings Volume 6343, Photonics North 2006; 634340 (2006) https://doi.org/10.1117/12.708257
Event: Photonics North 2006, 2006, Quebec City, Canada

Abstract

In a complex field such as bio-molecular diagnostics it is significant to know the behaviour of molecules in each assay before they are available for real time testing on devices. With numerous deadly diseases around mankind, behaviour of bio-molecules associated with each of these diseases becomes a first priority for the molecular diagnostics. The purpose of this review is to highlight the behaviour of enzymatic molecules using vibrations in conjunction with Micro-Electro-Mechanical Systems (MEMS) structures, which can be used as a diagnostic tool in a rapidly growing field of medical discipline. The authors introduce piezo-electric actuators in the experimental set-up that is used to accelerate the enzymatic molecular reaction in minute quantities of the reactant. The discussion for the above method is well supported by a body of literature from both biomedical and mechanical engineering. These enzymes are made to interact with their respective anti-bodies, whose reaction can be detected using many methods among which fluorescence spectroscopy is of preference and further detected using specific MEMS structures whose changes are detected through optical means. The results described here are intended to give a methodological approach to the creation of device in the future in the medical field for the detection of bio-molecules. Experimental results include pictures taken during shaking with piezo-electric stacks; fluorescence spectrometer results confirming a reaction between the enzyme and its anti-body and the biochemical reaction-taking place on the surface of the cantilever beams. The intent of this review is to better understand the behaviour of an enzymatic molecule under the influence of vibrations. With insight into the principles underlying the operation of the vibration experiment, the results could help to evaluate the value of a device that could be used in the molecular diagnostics in a simpler, faster and less expensive way.

Citation Download Citation

Amritsar Jeetender, Ion Stiharu, Muthukumaran Packirisamy, and Vahé Nerguizian "Behaviour of enzyme molecules under piezo-electric vibrations", Proc. SPIE 6343, Photonics North 2006, 634340 (8 September 2006); https://doi.org/10.1117/12.708257

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