Serial endoscopy in azoxymethane treated mice using ultra-high resolution optical coherence tomography

Lida P. Hariri; Ziping Qiu; Alexandre R. Tumlinson; David G. Besselsen; Eugene W. Gerner; Natalia Ignatenko; Boris Považay; Boris Hermann; Harald Sattmann; James McNally; Angelika Unterhuber; Wolfgang Drexler; Jennifer K. Barton

doi:10.1117/12.699249

13 February 2007 Serial endoscopy in azoxymethane treated mice using ultra-high-resolution optical coherence tomography

Lida P. Hariri, Ziping Qiu, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Natalia Ignatenko, Boris Považay, Boris Hermann, Harald Sattmann, James McNally, Angelika Unterhuber, Wolfgang Drexler, Jennifer K. Barton

Author Affiliations +

Proceedings Volume 6432, Endoscopic Microscopy II; 643208 (2007) https://doi.org/10.1117/12.699249
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Purpose: Optical coherence tomography (OCT) is a minimally invasive, depth-resolved imaging tool that can be commissioned for small diameter endoscopic applications for imaging mouse models of colorectal cancer. In this study, we utilized ultrahigh resolution OCT (UHR OCT) to serially image the lower colon of azoxymethane (AOM) treated A/J mouse models of CRC, monitor the progression of neoplastic transformations, and determine if OCT is capable of identifying early disease. Experimental Design: Thirteen AOM treated A/J and two control A/J mice were surveyed at four timepoints (8, 14, 22, and 26 weeks post AOM treatment) using a prototype 2.0 mm diameter UHR OCT endoscope-based system that achieved resolutions of 3.2 um axial and 4.4 um lateral. Histological samples were obtained at the final imaging timepoint serving as the gold standard. Results: Gross and histological assessment of the excised colonic tissue revealed at least one tumor in all 13 AOM treated mice, with most mice developing multiple tumors. In the corresponding OCT images, a progression from healthy thin mucosa to adenoma appearing as large, structurally disorganized masses was visualized over the imaging time points correlating to the locations of the grossly visualized tumors. Conclusions: This study indicates that UHR OCT enables accurate identification of disease and non-destructive visualization of CRC progression in the lower colon of mice.

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Lida P. Hariri, Ziping Qiu, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Natalia Ignatenko, Boris Považay, Boris Hermann, Harald Sattmann, James McNally, Angelika Unterhuber, Wolfgang Drexler, and Jennifer K. Barton "Serial endoscopy in azoxymethane treated mice using ultra-high-resolution optical coherence tomography", Proc. SPIE 6432, Endoscopic Microscopy II, 643208 (13 February 2007); https://doi.org/10.1117/12.699249

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