Multiplexed and Quantitative Study of Biomarker Expression in Tumor Specimens Using Quantum Dots

Aileen Wu; Lawrence True; Xiaohu Gao

doi:10.1117/12.661783

27 March 2006 Multiplexed and quantitative study of biomarker expression in tumor specimens using quantum dots

Aileen Wu, Lawrence True, Xiaohu Gao

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60961C (2006) https://doi.org/10.1117/12.661783
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

When conjugated with targeting molecules, quantum dots (QD) can be used as powerful cancer diagnostic tools providing the molecular profiles of cancer cases based on common clinical biopsies. Such personalized analyses will enable doctors to treat and manage the patients' diseases more effectively. The unique optical properties (e.g., size-tunable emission, simultaneous excitation, high brightness and photostability) of these nanoparticles make them superior to conventionally popular organic fluorophores^1-2. Polymer-encapsulated, antibody-tagged QDs were prepared and used to successfully stain both fixed and live cells as well as clinical formalin-fixed paraffin-embedded (FFPE) tissue sections. In the tissue staining study, QD bioconjugates targeting mutated p53 and early growth response protein (egr-1) were used to examine prostate cancer tissues. The tissue slides were then analyzed with a wavelength-resolved spectrometer to accurately quantify the protein expression levels. In comparison to traditional qualitatively based diagnostic procedures, quantum dot nanotechnology allows for a more quantitative, rigorous and objective analysis of tissue specimens in question. In addition, new developments in imaging instrumentation could automate spectroscopy measurements and data analysis.

Citation Download Citation

Aileen Wu, Lawrence True, and Xiaohu Gao "Multiplexed and quantitative study of biomarker expression in tumor specimens using quantum dots", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60961C (27 March 2006); https://doi.org/10.1117/12.661783

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available