An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses

W. Supatto; D. Débarre; B. Moulia; E. Farge; E. Beaurepaire

doi:10.1117/12.645897

22 February 2006 An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses

W. Supatto, D. Débarre, B. Moulia, E. Farge, E. Beaurepaire

Author Affiliations +

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 60881D (2006) https://doi.org/10.1117/12.645897
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The regulation of morphogenetic movements that shape an embryo during its development remains a challenging issue in developmental biology, and may in certain cases involve mechanical sensitivity. Addressing this issue requires novel experimental approaches. We show that the combination of femtosecond laser pulse-induced ablation and multiphoton microcopy can be used to modulate and quantify morphogenetic movements in Drosophila embryos. We characterized the effects of focused nanoJoule pulse trains in developing embryos. We used targeted ablations to locally modify the embryo structural integrity and modulate morphogenesis. Femtosecond-pulse induced ablation was combined with nonlinear microscopy based on two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG).Correlation-based analysis of microscopy data allowed us to track the outcome of ablations and to analyze tissue deformations. These experiments provided insight into the interplay between gene expression and tissue deformations in developing embryos.

Citation Download Citation

W. Supatto, D. Débarre, B. Moulia, E. Farge, and E. Beaurepaire "An all-optical approach to modulate and quantitatively analyse embryo morphogenetic movements by using ultrashort laser pulses", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881D (22 February 2006); https://doi.org/10.1117/12.645897

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available