Frame self-division applied in analysis of intrinsic signal optical imaging data

Weihua Luo; Pengcheng Li; Li Zhang; Xiaohua Lv; Shaoqun Zeng; Qingming Luo

doi:10.1117/12.645570

3 March 2006 Frame self-division applied in analysis of intrinsic signal optical imaging data

Weihua Luo, Pengcheng Li, Li Zhang, Xiaohua Lv, Shaoqun Zeng, Qingming Luo

Proceedings Volume 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III; 60850E (2006) https://doi.org/10.1117/12.645570
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In intrinsic signal optical imaging data, the response signal related cortical activity is very small compared to the background signal intensity and accompanied with unwanted noise from several different sources. Although there are some sophisticated data analysis techniques developed to achieve this critical demand, experience proved that no method perfect enough to be optimal for all experimental situations. Here we introduced a simple and practicable pre-processing method to attenuate the global noise source, such as the fluctuation of the illuminating light, changes of the animal's blood pressure and vasomotor oscillations. Before image processing to characterize the spatiotemporal response from cortical activity, each frame of image data was divided by the average or median of the current frame data. We applied the method to both simulated data and experimental optical imaging data to evaluate the validity and limitation.

Citation Download Citation

Weihua Luo, Pengcheng Li, Li Zhang, Xiaohua Lv, Shaoqun Zeng, and Qingming Luo "Frame self-division applied in analysis of intrinsic signal optical imaging data", Proc. SPIE 6085, Complex Dynamics and Fluctuations in Biomedical Photonics III, 60850E (3 March 2006); https://doi.org/10.1117/12.645570

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KEYWORDS

Optical imaging

Interference (communication)

Signal to noise ratio

Image processing

Data processing

Biomedical optics

Signal analyzers

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