Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska; Evgenia G. Matveeva; Ignacy Gryczynski; Robert W. Flower; Joanna Malicka; Joseph R. Lakowicz; Slawomir Makowiec; Zygmunt K. Gryczynski

doi:10.1117/12.593426

31 March 2005 Enhanced fluorescence of labeled proteins coated on silver nanoparticles

Joanna Lukomska, Evgenia G. Matveeva, Ignacy Gryczynski, Robert W. Flower, Joanna Malicka, Joseph R. Lakowicz, Slawomir Makowiec, Zygmunt K. Gryczynski

Author Affiliations +

Proceedings Volume 5703, Plasmonics in Biology and Medicine II; (2005) https://doi.org/10.1117/12.593426
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

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Joanna Lukomska, Evgenia G. Matveeva, Ignacy Gryczynski, Robert W. Flower, Joanna Malicka, Joseph R. Lakowicz, Slawomir Makowiec, and Zygmunt K. Gryczynski "Enhanced fluorescence of labeled proteins coated on silver nanoparticles", Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); https://doi.org/10.1117/12.593426

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