Fluorescence of prostate-specific antigen as measured with a portable 1D scanner

Byeong C. Kim; Jin H. Jeong; Dong S. Jeong; Young M. Kim; Sang W. Oh; Eui Y. Choi; Jae H. Kim; Kie Bong Nahm

doi:10.1117/12.577226

18 January 2005 Fluorescence of prostate-specific antigen as measured with a portable 1D scanner

Byeong C. Kim, Jin H. Jeong, Dong S. Jeong, Young M. Kim, Sang W. Oh, Eui Y. Choi, Jae H. Kim, Kie Bong Nahm

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.577226
Event: Photonics Asia, 2004, Beijing, China

Abstract

Prostate-specific antigen (PSA) is an androgen-dependent glycoprotein protease (M.W. 33 kDa) and a member of kallikrein super-family of serine protease, and has chymotrypsin-like enzymatic activity. It is synthesized by the prostate epithelial cells and found in the prostate gland and seminal plasma as a major protein. It is widely used as a clinical marker for diagnosis, screening, monitoring and prognosis of prostate cancer. In normal male adults, the concentration of PSA in the blood is below 4 ng/ml and this value increases in patients with the prostate cancer or the benign prostatic hyperplasia (BPH) due to its leakage into the circulatory system. As such, systematic monitoring of the PSA level in the blood can provide critical information about the progress of the prostatic disease. We have developed a compact integral system that can quantitatively measure the concentration of total PSA in human blood. This system utilizes the fluorescence emitted from the dye molecules attached to PSA molecules after appropriate immunoassay-based processing. Developed for the purpose of providing an affordable means of fast point-of-care testing of the prostate cancer, this system proved to be able to detect the presence of the PSA at the level of 0.18 ng/ml in less than 12 minutes, with the actual measurement taking less than 2 minutes. The design concept for this system is presented together with the result for a few representative samples.

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Byeong C. Kim, Jin H. Jeong, Dong S. Jeong, Young M. Kim, Sang W. Oh, Eui Y. Choi, Jae H. Kim, and Kie Bong Nahm "Fluorescence of prostate-specific antigen as measured with a portable 1D scanner", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.577226

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KEYWORDS

Luminescence

Blood

Molecules

Optical filters

Prostate

Prostate cancer

Proteins

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