Imaging of interferences between cellular particles and the fluorescent dye Merocyanine-540 during normoxia and anoxia

Christine Mahlke; Marc Dammann; Valerie Steinbach; Maria-Theresa Stingl; Manfred D. Kessler

doi:10.1117/12.469466

5 June 2002 Imaging of interferences between cellular particles and the fluorescent dye Merocyanine-540 during normoxia and anoxia

Christine Mahlke, Marc Dammann, Valerie Steinbach, Maria-Theresa Stingl, Manfred D. Kessler

Author Affiliations +

Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469466
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Subcellular particles (mitochondria, cellular pigments etc.) are mainly responsible for light scattering in living tissues in relation to the functional state. 2-D images clarify the respective tissue status during normoxia or anoxia such as the redox state of cytochromes. We realized tissue imaging of twelve isolated perfused rat livers stained with Merocyanine-540 during normoxia, hypoxia and anoxia. Merocyanine-spectra have shown a maximum fluorescence peak at 596 +/- 2 nm. The optical response increased under desoxygenation and decreased under reoxygenation and might be correlated to electrical potential alterations. Furthermore, we record oscillations with frequencies over 7/sec (420/min) which might be correlated to intracellular processes. The additional use of dyes for tissue imaging gives us the opportunity of new insights into organ function.

Citation Download Citation

Christine Mahlke, Marc Dammann, Valerie Steinbach, Maria-Theresa Stingl, and Manfred D. Kessler "Imaging of interferences between cellular particles and the fluorescent dye Merocyanine-540 during normoxia and anoxia", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469466

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