Catchment volume and penetration depth in different suspensions of liver mitochondria monitored in scattering chambers

Marc Dammann; Christine Mahlke; Valerie Steinbach; Michael Singer; Manfred D. Kessler

doi:10.1117/12.469440

5 June 2002 Catchment volume and penetration depth in different suspensions of liver mitochondria monitored in scattering chambers

Marc Dammann, Christine Mahlke, Valerie Steinbach, Michael Singer, Manfred D. Kessler

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Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469440
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Spectrometry is a well established method for in vitro and in vivo measurements. For interpretation of experimental results and patient monitoring it is important to regard the catchment volume and penetration depth of backscattered light. We investigated different suspensions of rat liver mitochondria as well as rat liver homogenate in a scattering chamber combined with a scanning procedure. To compare these results with a common used medium we measured Lipofundin 2% additionally. The highest light intensity caused Lipofundin 2%, followed by 100% mitochondrial concentration, liver homogenate and 50% mitochondrial concentration. Most of times the light penetrates slightly pronounced in lateral than in forward direction. Furthermore, we investigated the dependence of optical fibers with different thickness on catchment volume and penetration depth. Optical fibers were applied with a diameter of 200 micrometers and 250 micrometers respectively presenting a great increase in catchment volume and penetration depth in the larger one.

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Marc Dammann, Christine Mahlke, Valerie Steinbach, Michael Singer, and Manfred D. Kessler "Catchment volume and penetration depth in different suspensions of liver mitochondria monitored in scattering chambers", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469440

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KEYWORDS

Liver

Optical fibers

Scattering

Light scattering

Tissue optics

Particles

Absorption

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