Hydrodynamic activation and sorting of white blood cells in a microfabricated lattice

Robert H. Carlson; Christopher V. Gabel; Shirley S. Chan; Robert H. Austin; James P. Brody; James W. Winkelman M.D.

doi:10.1117/12.269970

31 March 1997 Hydrodynamic activation and sorting of white blood cells in a microfabricated lattice

Robert H. Carlson, Christopher V. Gabel, Shirley S. Chan, Robert H. Austin, James P. Brody, James W. Winkelman M.D.

Proceedings Volume 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications; (1997) https://doi.org/10.1117/12.269970
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We demonstrate a novel hydrodynamic shear activation of leucocyte adhesion, using physiological flow conditions and a microfabricated array of channels with length scales similar to those of human capillaries. Vital chromosome stains and cell specific fluorochrome labeled antibodies reveal that the eventual adhesion of the leukocytes to the silicon array displays a strong dependence on cell type and nuclear morphology, with granulocytes activating more rapidly with distance and penetrating a smaller distance than lymphocytes. Further, the granulocytes interact with the lymphocytes in a self-exclusionary manner under shearing flow with the eventual separation of the two cell types in the array. Such arrays of microfabricated obstacles thus have an interesting potential for sorting white blood cells by type from a 10 microliter drop of whole blood.

Citation Download Citation

Robert H. Carlson, Christopher V. Gabel, Shirley S. Chan, Robert H. Austin, James P. Brody, and James W. Winkelman M.D. "Hydrodynamic activation and sorting of white blood cells in a microfabricated lattice", Proc. SPIE 2978, Micro- and Nanofabricated Electro-Optical Mechanical Systems for Biomedical and Environmental Applications, (31 March 1997); https://doi.org/10.1117/12.269970

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