Multiphoton microscopy of ECM proteins in baboon aortic leaflet

Mariacarla Gonzalez; Ilyas Saytashev; Camila Luna; Brittany Gonzalez; Alejandro Pinero; Manuel Perez; Sharan Ramaswamy; Jessica Ramella-Roman

doi:10.1117/12.2291024

22 February 2018 Multiphoton microscopy of ECM proteins in baboon aortic leaflet

Mariacarla Gonzalez, Ilyas Saytashev, Camila Luna, Brittany Gonzalez, Alejandro Pinero, Manuel Perez, Sharan Ramaswamy, Jessica Ramella-Roman

Author Affiliations +

Proceedings Volume 10471, Diagnostic and Therapeutic Applications of Light in Cardiology 2018; 104710Y (2018) https://doi.org/10.1117/12.2291024
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The extracellular matrix (ECM) plays crucial role in defining mechanical properties of a heart valve yet the mechanobiological role of the ECM proteins – collagen and elastin - in living heart valve leaflets is still poorly understood. In this study, non-linear microscopy was used to obtain three dimensional images of collagen and elastin arrangement in aortic leaflets under combined steady flow (850 ml/min) and cyclic flexure (1 Hz) mechanical (dynamic) training. A novel bioreactor capable of mimicking the flow conditions in a living heart was used in this study and was optimized for microscopic imagery. A custom made non-linear microscope was used in this study to provide Second Harmonic Generation (SHG) imaging of collagen arrangement and two-photon imaging of elastin. Two control and three trained leaflet samples from static and dynamic tissue culture were imaged to observe protein changes in the tissue for a period of seven days. Dynamic training led to a decrease in alignment index of the protein fibers compared to the static treatment.

Citation Download Citation

Mariacarla Gonzalez, Ilyas Saytashev, Camila Luna, Brittany Gonzalez, Alejandro Pinero, Manuel Perez, Sharan Ramaswamy, and Jessica Ramella-Roman "Multiphoton microscopy of ECM proteins in baboon aortic leaflet", Proc. SPIE 10471, Diagnostic and Therapeutic Applications of Light in Cardiology 2018, 104710Y (22 February 2018); https://doi.org/10.1117/12.2291024

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